The Neff strain of Acanthamoeba castellanii, a tool for testing the virulence of Mycobacterium kansasii.

Details

Serval ID
serval:BIB_C35615F8CA4E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The Neff strain of Acanthamoeba castellanii, a tool for testing the virulence of Mycobacterium kansasii.
Journal
Research In Microbiology
Author(s)
Goy G., Thomas V., Rimann K., Jaton K., Prod'hom G., Greub G.
ISSN
0923-2508 (Print)
ISSN-L
0923-2508
Publication state
Published
Issued date
2007
Volume
158
Number
4
Pages
393-397
Language
english
Notes
Publication types: Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Virulent Mycobacterium kansasii (mainly subtype 1) may cause lung infections, whereas certain other strains (essentially subtype 3) are commonly non-pathogenic mycobacteria colonizing the human lower respiratory tract of patients. Determining the clinical significance of a strain isolated from a respiratory sample represents a major challenge for clinicians. Since some mycobacteria may use free-living amoebae as a training ground to select virulence traits, we wondered whether the Acanthamoeba castellanii amoeba could be used to determine the virulence of these intracellular bacteria. We investigated whether the growth and cytopathic effect of M. kansasii in A. castellanii correlate with the virulence of M. kansasii determined clinically and by subtyping. Pathogenic subtype 1 M. kansasii strains grew better in A. castellanii than non-pathogenic subtype 3 strains when considering both the number of bacteria per amoeba and the percentage of infected amoebae. Moreover, a subtype 3 M. kansasii strain isolated from blood culture, and thus considered pathogenic, was revealed to grow in A. castellanii similarly to pathogenic subtype 1 strains. These results suggest that amoebae may represent useful tools for testing the virulence of intracellular mycobacteria and other amoeba-resisting bacteria. This is important, since identification of novel bacterial virulence factors relies largely on in vitro assessment of virulence.
Keywords
Acanthamoeba castellanii/microbiology, Animals, Bacterial Typing Techniques, Humans, Mycobacterium kansasii/classification, Mycobacterium kansasii/growth & development, Virulence
Pubmed
Web of science
Create date
25/01/2008 14:28
Last modification date
20/08/2019 15:38
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