Analysis of the contribution of the β-oxidation auxiliary enzymes in the degradation of the dietary conjugated linoleic acid 9-cis-11-trans-octadecadienoic acid in the peroxisomes of Saccharomyces cerevisiae

Details

Serval ID
serval:BIB_C2D7540A94F8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Analysis of the contribution of the β-oxidation auxiliary enzymes in the degradation of the dietary conjugated linoleic acid 9-cis-11-trans-octadecadienoic acid in the peroxisomes of Saccharomyces cerevisiae
Journal
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
Author(s)
Bogdawa Heique, Delessert Syndie, Poirier Yves
ISSN
1388-1981
ISSN-L
1879-2618
Publication state
Published
Issued date
08/2005
Peer-reviewed
Oui
Volume
1735
Number
3
Pages
204-213
Language
english
Abstract
Beta-oxidation of the conjugated linoleic acid 9-cis,11-trans-octadecadienoic acid (rumenic acid) was analyzed in vivo in Saccharomyces cerevisiae by monitoring polyhydroxyalkanoate production in the peroxisome. Polyhydroxyalkanoate is synthesized by the polymerization of the beta-oxidation intermediates 3-hydroxyacyl-CoAs via a bacterial polyhydroxyalkanoate synthase targeted to the peroxisome. The amount of polyhydroxyalkanaote synthesized from the degradation of rumenic acid was found to be similar to the amount synthesized from the degradation of 10-trans,12-cis-octadecadienoic acid, oleic acid or 10-cis-heptadecenoic acid. Furthermore, the degradation of 10-cis-heptadecenoic acid was found to be unaffected by the presence of rumenic acid in the media. Efficient degradation of rumenic acid was found to be independent of the Delta(3,5),Delta(2,4)-dienoyl-CoA isomerase but instead relied on the presence of Delta(3),Delta(2)-enoyl-CoA isomerase activity. The presence of the unsaturated monomer 3-hydroxydodecenoic acid in polyhydroxyalkanoate derived from rumenic acid degradation was found to be dependent on the presence of a Delta(3),Delta(2)-enoyl-CoA isomerase activity. Together, these data indicate that rumenic acid is mainly degraded in vivo in S. cerevisiae through a pathway requiring only the participation of the auxiliary enzymes Delta(3),Delta(2)-enoyl-CoA isomerase, along with the enzyme of the core beta-oxidation cycle.
Keywords
Linoleic Acids/metabolism, Linoleic Acids, Conjugated/metabolism, Oxidation-Reduction, Peroxisomes/enzymology, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/metabolism, Cell Biology, Molecular Biology
Pubmed
Web of science
Create date
27/05/2010 10:28
Last modification date
20/08/2019 16:38
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