High commitment of embryonic keratinocytes to terminal differentiation through a Notch1-caspase 3 regulatory mechanism.

Details

Serval ID
serval:BIB_C27F81F93AE8
Type
Article: article from journal or magazin.
Collection
Publications
Title
High commitment of embryonic keratinocytes to terminal differentiation through a Notch1-caspase 3 regulatory mechanism.
Journal
Developmental Cell
Author(s)
Okuyama R., Nguyen B.C., Talora C., Ogawa E., Tommasi di Vignano A., Lioumi M., Chiorino G., Tagami H., Woo M., Dotto G.P.
ISSN
1534-5807 (Print)
ISSN-L
1534-5807
Publication state
Published
Issued date
2004
Volume
6
Number
4
Pages
551-562
Language
english
Abstract
Embryonic cells are expected to possess high growth/differentiation potential, required for organ morphogenesis and expansion during development. However, little is known about the intrinsic properties of embryonic epithelial cells due to difficulties in their isolation and cultivation. We report here that pure keratinocyte populations from E15.5 mouse embryos commit irreversibly to differentiation much earlier than newborn cells. Notch signaling, which promotes keratinocyte differentiation, is upregulated in embryonic keratinocyte and epidermis, and elevated caspase 3 expression, which we identify as a transcriptional Notch1 target, accounts in part for the high commitment of embryonic keratinocytes to terminal differentiation. In vivo, lack of caspase 3 results in increased proliferation and decreased differentiation of interfollicular embryonic keratinocytes, together with decreased activation of PKC-delta, a caspase 3 substrate which functions as a positive regulator of keratinocyte differentiation. Thus, a Notch1-caspase 3 regulatory mechanism underlies the intrinsically high commitment of embryonic keratinocytes to terminal differentiation.
Keywords
Animals, Animals, Newborn, Caspase 3, Caspases/genetics, Caspases/metabolism, Cell Differentiation/genetics, Cell Lineage/genetics, Cells, Cultured, Epidermis/cytology, Epidermis/embryology, Fetus, Keratinocytes/cytology, Keratinocytes/metabolism, Mice, Protein Kinase C/genetics, Protein Kinase C/metabolism, Protein Kinase C-delta, Receptor, Notch1, Receptors, Cell Surface/genetics, Receptors, Cell Surface/metabolism, Signal Transduction/drug effects, Signal Transduction/physiology, Transcription Factors, Up-Regulation/genetics
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:58
Last modification date
20/08/2019 15:37
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