ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans.

Details

Serval ID
serval:BIB_C246876CE4FD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans.
Journal
The Journal of antimicrobial chemotherapy
Author(s)
Kieffer N., Guzmán-Puche J., Poirel L., Kang H.J., Jeon C.O., Nordmann P.
ISSN
1460-2091 (Electronic)
ISSN-L
0305-7453
Publication state
Published
Issued date
01/06/2019
Peer-reviewed
Oui
Volume
74
Number
6
Pages
1568-1571
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Our aim was to characterize the putative MBL of the environmental strain Zhongshania aliphaticivorans isolated from a marine environment.
The putative MBL was identified in silico using the NCBI database. The β-lactamase gene was cloned into different Escherichia coli backgrounds. Kinetic parameters were determined using the purified enzyme.
The enzyme named ZHO-1 shared 51% amino acid identity with the acquired class B carbapenemases IMP-1, KHM-1 and DIM-1. Expression of the blaZHO-1 gene in a susceptible E. coli resulted in a carbapenemase phenotype. Kinetic parameters determined from purified ZHO-1 enzyme showed that it had significant hydrolytic activity against most β-lactams including penicillins, cephalosporins and carbapenems, with the exception of aztreonam and cefepime.
This study adds to the knowledge regarding environmental species as a reservoir of possible clinically relevant MBLs.
Keywords
Amino Acid Sequence, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/chemistry, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Cloning, Molecular, Drug Resistance, Multiple, Bacterial/genetics, Gammaproteobacteria/drug effects, Gammaproteobacteria/enzymology, Gene Expression Regulation, Bacterial, beta-Lactamases/genetics, beta-Lactamases/metabolism
Pubmed
Web of science
Create date
31/03/2019 15:11
Last modification date
25/07/2020 6:19
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