Real-time evaluation of macozinone activity against Mycobacterium tuberculosis through bacterial nanomotion analysis.

Details

Serval ID
serval:BIB_C1A51AF18AEE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Real-time evaluation of macozinone activity against Mycobacterium tuberculosis through bacterial nanomotion analysis.
Journal
Antimicrobial agents and chemotherapy
Author(s)
Vocat A., Luraschi-Eggemann A., Antoni C., Cathomen G., Cichocka D., Greub G., Riabova O., Makarov V., Opota O., Mendoza A., Cole S.T., Sturm A.
ISSN
1098-6596 (Electronic)
ISSN-L
0066-4804
Publication state
In Press
Peer-reviewed
Oui
Language
english
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Abstract
Novel drugs and improved diagnostics for Mycobacterium tuberculosis (MTB) are urgently needed and go hand in hand. We evaluated the in vitro activity of two benzothiazinone drug candidates (MCZ, PBTZ169; BTZ043) and their main metabolites against MTB using advanced nanomotion technology. The results demonstrated significant reductions in MTB viability within 7 h, indicating the potential for rapid, precise antibiotic susceptibility testing based on a phenotypic read-out in real time. PBTZ169 and H <sub>2</sub> -PBTZ169 achieved 100% separation between the susceptible H37Rv and a resistant dprE1 mutant strain NTB1. These findings support nanomotion technology's potential for faster antibiotic susceptibility testing of novel MTB drug candidates targeting the DprE1 enzyme that could reduce empirical treatment duration and antibiotic resistance selection pressure due to inaccurate treatments.
Keywords
DprE1 inhibitors, Mycobacterium tuberculosis, antibiotic susceptibility test, macozinone, nanomotion
Pubmed
Web of science
Create date
02/12/2024 14:29
Last modification date
20/12/2024 7:07
Usage data