Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization.

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Version: author
Serval ID
serval:BIB_C182D6166CED
Type
Article: article from journal or magazin.
Collection
Publications
Title
Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization.
Journal
Molecular Cell
Author(s)
Diebold-Durand M.L., Lee H., Ruiz Avila L.B., Noh H., Shin H.C., Im H., Bock F.P., Bürmann F., Durand A., Basfeld A., Ham S., Basquin J., Oh B.H., Gruber S.
ISSN
1097-4164 (Electronic)
ISSN-L
1097-2765
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
67
Number
2
Pages
334-347.e5
Language
english
Abstract
Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain connected by a 49 nm coiled-coil "arm." The heads undergo ATP-dependent engagement and disengagement to drive SMC action on the chromosome. Here, we elucidate the architecture of prokaryotic Smc dimers by high-throughput cysteine cross-linking and crystallography. Co-alignment of the Smc arms tightly closes the interarm space and misaligns the Smc head domains at the end of the rod by close apposition of their ABC signature motifs. Sandwiching of ATP molecules between Smc heads requires them to substantially tilt and translate relative to each other, thereby opening up the Smc arms. We show that this mechanochemical gating reaction regulates chromosome targeting and propose a mechanism for DNA translocation based on the merging of DNA loops upon closure of Smc arms.

Keywords
DNA loop extrusion, MukB, Rad50, SMC, ScpA, ScpB, chromosome condensation, cohesion, condensin, kite, kleisin
Pubmed
Web of science
Open Access
Yes
Create date
12/07/2017 10:37
Last modification date
20/08/2019 16:36
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