Hepatic gene expression profiling in Nrf2 knockout mice after long-term high-fat diet-induced obesity.

Details

Serval ID
serval:BIB_C12D65A41242
Type
Article: article from journal or magazin.
Collection
Publications
Title
Hepatic gene expression profiling in Nrf2 knockout mice after long-term high-fat diet-induced obesity.
Journal
Oxidative Medicine and Cellular Longevity
Author(s)
Chartoumpekis D.V., Ziros P.G., Zaravinos A., Iskrenova R.P., Psyrogiannis A.I., Kyriazopoulou V.E., Sykiotis G.P., Habeos I.G.
ISSN
1942-0994 (Electronic)
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
2013
Pages
340731
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
INTRODUCTION: The transcription factor NFE2-related factor 2 (Nrf2) is a central regulator of antioxidant and detoxification gene expression in response to electrophilic or oxidative stress. Nrf2 has recently been shown to cross-talk with metabolic pathways, and its gene deletion protected mice from high-fat-diet-(HFD-) induced obesity and insulin resistance. This study aimed to identify potential Nrf2-regulated genes of metabolic interest by comparing gene expression profiles of livers of wild-type (WT) versus Nrf2 knockout (Nrf2-KO) mice after a long-term HFD.
METHODS: WT and Nrf2-KO mice were fed an HFD for 180 days; total RNA was prepared from liver and used for microarray analysis and quantitative real-time RT-PCR (qRT-PCR).
RESULTS: The microarray analysis identified 601 genes that were differentially expressed between WT and Nrf2-KO mice after long-term HFD. Selected genes, including ones known to be involved in metabolic regulation, were prioritized for verification by qRT-PCR: Cyp7a1 and Fabp5 were significantly overexpressed in Nrf2-KO mice; in contrast, Car, Cyp2b10, Lipocalin 13, Aquaporin 8, Cbr3, Me1, and Nqo1 were significantly underexpressed in Nrf2-KO mice.
CONCLUSION: Transcriptome profiling after HFD-induced obesity confirms that Nrf2 is implicated in liver metabolic gene networks. The specific genes identified here may provide insights into Nrf2-dependent mechanisms of metabolic regulation.
Keywords
Animals, Diet, High-Fat/adverse effects, Female, Gene Expression Profiling, Gene Expression Regulation, Gene Ontology, Gene Regulatory Networks, Liver/metabolism, Liver/pathology, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, NF-E2-Related Factor 2/deficiency, NF-E2-Related Factor 2/metabolism, Obesity/genetics, Obesity/pathology, Oligonucleotide Array Sequence Analysis, RNA, Messenger/genetics, RNA, Messenger/metabolism, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction/drug effects, Signal Transduction/genetics, Time Factors
Pubmed
Web of science
Open Access
Yes
Create date
20/01/2015 14:38
Last modification date
20/08/2019 16:35
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