Altered proliferation and differentiation of human epidermis in cases of skin fibrosis after radiotherapy.
Details
Serval ID
serval:BIB_C118D06891C4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Altered proliferation and differentiation of human epidermis in cases of skin fibrosis after radiotherapy.
Journal
International journal of radiation oncology, biology, physics
ISSN
0360-3016 (Print)
ISSN-L
0360-3016
Publication state
Published
Issued date
01/06/2002
Peer-reviewed
Oui
Volume
53
Number
2
Pages
385-393
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
To characterize, at the histopathologic and molecular levels, the irradiated epidermis in cases of human skin fibrosis induced by radiotherapy.
Surgical samples were obtained from 6 patients who had developed cutaneous fibronecrotic lesions from 7 months to 27 years after irradiation. The proliferation and differentiation status of the irradiated epidermis was characterized with specific markers using immunohistochemical methods.
All samples presented with hyperplasia of the epidermis associated with local inflammation. The scar epidermis exhibited an increased expression of proliferating cell nuclear antigen, which revealed hyperproliferation of keratinocytes. Furthermore, an abnormal differentiation was found, characterized by the expression of K6 and K16, and by alterations in protein amounts and localization of cytokeratins, involucrin, and transforming growth factor-beta1.
These results demonstrate that late damage of irradiated skin is not only characterized by fibrosis in the dermis but also by hyperplasia in the epidermis. This hyperplasia was due to both hyperproliferation and abnormal differentiation of keratinocytes.
Surgical samples were obtained from 6 patients who had developed cutaneous fibronecrotic lesions from 7 months to 27 years after irradiation. The proliferation and differentiation status of the irradiated epidermis was characterized with specific markers using immunohistochemical methods.
All samples presented with hyperplasia of the epidermis associated with local inflammation. The scar epidermis exhibited an increased expression of proliferating cell nuclear antigen, which revealed hyperproliferation of keratinocytes. Furthermore, an abnormal differentiation was found, characterized by the expression of K6 and K16, and by alterations in protein amounts and localization of cytokeratins, involucrin, and transforming growth factor-beta1.
These results demonstrate that late damage of irradiated skin is not only characterized by fibrosis in the dermis but also by hyperplasia in the epidermis. This hyperplasia was due to both hyperproliferation and abnormal differentiation of keratinocytes.
Keywords
Adult, Aged, Cell Differentiation/radiation effects, Cell Division/radiation effects, Cicatrix/metabolism, Cicatrix/pathology, Female, Humans, Hyperplasia/etiology, Hyperplasia/metabolism, Hyperplasia/pathology, Integrins/metabolism, Keratinocytes/metabolism, Keratinocytes/pathology, Keratinocytes/radiation effects, Keratins/metabolism, Male, Middle Aged, Proliferating Cell Nuclear Antigen/metabolism, Radiation Injuries/metabolism, Radiation Injuries/pathology, Skin/metabolism, Skin/pathology, Skin/radiation effects, Transforming Growth Factor beta/metabolism
Pubmed
Web of science
Create date
27/04/2018 16:26
Last modification date
20/08/2019 16:35
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