The salivary microbiome for differentiating individuals: proof of principle.
Details
State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_C1090D3E0E19
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The salivary microbiome for differentiating individuals: proof of principle.
Journal
Microbes and infection
ISSN
1769-714X (Electronic)
ISSN-L
1286-4579
Publication state
Published
Issued date
06/2016
Peer-reviewed
Oui
Volume
18
Number
6
Pages
399-405
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Human identification has played a prominent role in forensic science for the past two decades. Identification based on unique genetic traits is driving the field. However, this may have limitations, for instance, for twins. Moreover, high-throughput sequencing techniques are now available and may provide a high amount of data likely useful in forensic science. This study investigates the potential for bacteria found in the salivary microbiome to be used to differentiate individuals. Two different targets (16S rRNA and rpoB) were chosen to maximise coverage of the salivary microbiome and when combined, they increase the power of differentiation (identification). Paired-end Illumina high-throughput sequencing was used to analyse the bacterial composition of saliva from two different people at four different time points (t = 0 and t = 28 days and then one year later at t = 0 and t = 28 days). Five major phyla dominate the samples: Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes and Fusobacteria. Streptococcus, a Firmicutes, is one of the most abundant aerobic genera found in saliva and targeting Streptococcus rpoB has enabled a deeper characterisation of the different streptococci species, which cannot be differentiated using 16S rRNA alone. We have observed that samples from the same person group together regardless of time of sampling. The results indicate that it is possible to distinguish two people using the bacterial microbiota present in their saliva.
Keywords
Adult, Bacteria/classification, Bacteria/genetics, Cluster Analysis, DNA, Bacterial/chemistry, DNA, Bacterial/genetics, DNA, Ribosomal/chemistry, DNA, Ribosomal/genetics, DNA-Directed RNA Polymerases, Forensic Anthropology/methods, Forensic Medicine/methods, Healthy Volunteers, High-Throughput Nucleotide Sequencing, Humans, Metagenomics/methods, Microbiota, Phylogeny, RNA, Ribosomal, 16S/genetics, Saliva/microbiology, Sequence Analysis, DNA
Pubmed
Create date
26/05/2016 11:02
Last modification date
20/08/2019 16:35