Immuno-electron microscopic identification of human estrogen receptor-DNA complexes at the estrogen-responsive element and in the first intron of a Xenopus vitellogenin gene.

Details

Serval ID
serval:BIB_C0EA63007232
Type
Article: article from journal or magazin.
Collection
Publications
Title
Immuno-electron microscopic identification of human estrogen receptor-DNA complexes at the estrogen-responsive element and in the first intron of a Xenopus vitellogenin gene.
Journal
Journal of Molecular Biology
Author(s)
ten Heggler-Bordier B., Claret F.X., Wahli W.
ISSN
0022-2836[print], 0022-2836[linking]
Publication state
Published
Issued date
11/1988
Volume
204
Number
1
Pages
217-220
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Using an extract of nuclei from the estrogen-responsive human breast cancer cell line MCF-7, protein-DNA complexes were assembled in vitro at the 5' end of the Xenopus laevis vitellogenin gene B2 that is normally expressed in liver after estrogen induction. The complexes formed were analyzed by electron microscopy after labeling by the indirect colloidal gold immunological method using a monoclonal antibody specific for the human estrogen receptor. As identified by its interaction with protein A-gold, the antibody was found linked to two protein-DNA complexes, the first localized at the estrogen responsive element of the gene and the second in intron I, thus proving a direct participation of the receptor in these two complexes. The procedure used allows the visualization and rapid localization of specific transcription factors bound in vitro to a promoter or any other gene region.
Keywords
Animals, Cell Line, DNA/analysis, Estrogens/metabolism, Genes, Humans, Immunohistochemistry, Introns, Macromolecular Substances, Microscopy, Electron, Receptors, Estrogen/analysis, Vitellogenins/genetics, Xenopus laevis/genetics
Pubmed
Web of science
Create date
24/01/2008 17:04
Last modification date
20/08/2019 16:35
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