Islet of Langerhans isolation from pediatric and juvenile donor pancreases.

Details

Serval ID
serval:BIB_C035310D9D81
Type
Article: article from journal or magazin.
Collection
Publications
Title
Islet of Langerhans isolation from pediatric and juvenile donor pancreases.
Journal
Transplant international
Author(s)
Meier R.P., Sert I., Morel P., Muller Y.D., Borot S., Badet L., Toso C., Bosco D., Berney T.
ISSN
1432-2277 (Electronic)
ISSN-L
0934-0874
Publication state
Published
Issued date
09/2014
Peer-reviewed
Oui
Volume
27
Number
9
Pages
949-955
Language
english
Notes
Publication types: Comparative Study ; Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Islet grafts isolated from young donors allow superior functional outcomes but are often associated with poor islet isolation yields. The objective of this study was to comparatively analyze the outcomes of islet isolation between young and older donors. We retrospectively analyzed 564 pancreas isolations performed at our institution. Isolation outcomes were compared between donors aged ≤20 years (n = 42, YD) and >20 years (n = 522, OD). Isolation procedure was identical in both groups. Prepurification percentage of embedded islets was higher in YD (44.3 ± 22.7% vs. 24.9 ± 20.9%, P < 0.001). This led to a lower recovery rate in YD (48% vs. 76%, P = 0.002) and hence lower postpurification IEQ/g pancreas in YD (2 412 ± 1 789 IEQ/g vs. 3 194 ± 1 892 IEQ/g, P = 0.01). Final yield was 180 982 ± 128 073 IEQ in YD and 244 167 ± 134 137 IEQ in OD, (P = 0.006). In vitro function was markedly, albeit nonsignificantly, higher in YD (SI: 4.5 ± 5.1 vs. 3.0 ± 5.7, P = 0.350). Proportion of transplanted preparations was similar in both groups, 38% (16/42) in YD vs. 43% (224/522) in OD, P = 0.628. In spite of isolation and purification difficulties, pancreases from young donors allowed similar islet transplantation rates as older donors. Efforts should be directed at improving islet extraction in these donors to realize their full potential for islet transplantation.
Keywords
Adolescent, Age Factors, Cause of Death, Cell Count, Cell Separation/instrumentation, Cell Separation/methods, Child, Cold Ischemia, Collagenases, Ficoll, Graft Survival, Humans, Islets of Langerhans/cytology, Islets of Langerhans Transplantation/methods, Islets of Langerhans Transplantation/statistics & numerical data, Pancreas/cytology, Retrospective Studies, Tissue Donors, Tissue and Organ Harvesting/methods, Treatment Outcome, Young Adult, islet isolation, islet transplantation, type 1 diabetes mellitus, young donors
Pubmed
Web of science
Create date
01/11/2023 15:09
Last modification date
13/04/2024 7:06
Usage data