Arginase activity in the blood of patients with visceral leishmaniasis and HIV infection.

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Serval ID
serval:BIB_C00EC508C2F9
Type
Article: article from journal or magazin.
Collection
Publications
Title
Arginase activity in the blood of patients with visceral leishmaniasis and HIV infection.
Journal
PLoS Neglected Tropical Diseases
Author(s)
Takele Y., Abebe T., Weldegebreal T., Hailu A., Hailu W., Hurissa Z., Ali J., Diro E., Sisay Y., Cloke T., Modolell M., Munder M., Tacchini-Cottier F., Müller I., Kropf P.
ISSN
1935-2735 (Electronic)
ISSN-L
1935-2727
Publication state
Published
Issued date
2013
Volume
7
Number
1
Pages
e1977
Language
english
Abstract
BACKGROUND: Visceral leishmaniasis is a parasitic disease associated with high mortality. The most important foci of visceral leishmaniasis in Ethiopia are in the Northwest and are predominantly associated with high rates of HIV co-infection. Co-infection of visceral leishmaniasis patients with HIV results in higher mortality, treatment failure and relapse. We have previously shown that arginase, an enzyme associated with immunosuppression, was increased in patients with visceral leishmaniasis and in HIV seropositive patients; further our results showed that high arginase activity is a marker of disease severity. Here, we tested the hypothesis that increased arginase activities associated with visceral leishmaniasis and HIV infections synergize in patients co-infected with both pathogens.
METHODOLOGY/PRINCIPAL FINDINGS: We recruited a cohort of patients with visceral leishmaniasis and a cohort of patients with visceral leishmaniasis and HIV infection from Gondar, Northwest Ethiopia, and recorded and compared their clinical data. Further, we measured the levels of arginase activity in the blood of these patients and identified the phenotype of arginase-expressing cells. Our results show that CD4(+) T cell counts were significantly lower and the parasite load in the spleen was significantly higher in co-infected patients. Moreover, our results demonstrate that arginase activity was significantly higher in peripheral blood mononuclear cells and plasma of co-infected patients. Finally, we identified the cells-expressing arginase in the PBMCs as low-density granulocytes.
CONCLUSION: Our results suggest that increased arginase might contribute to the poor disease outcome characteristic of patients with visceral leishmaniasis and HIV co-infection.
Keywords
Adolescent, Adult, Arginase/blood, Biological Markers/blood, Coinfection/diagnosis, Coinfection/pathology, Cross-Sectional Studies, Ethiopia, HIV Infections/complications, HIV Infections/diagnosis, Humans, Leishmaniasis, Visceral/complications, Leishmaniasis, Visceral/diagnosis, Male, Severity of Illness Index, Young Adult
Pubmed
Web of science
Open Access
Yes
Create date
13/03/2013 10:50
Last modification date
20/08/2019 16:34
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