Nuclear relocation of Kss1 contributes to the specificity of the mating response.

Details

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State: Public
Version: Final published version
Serval ID
serval:BIB_BFCC0FEC0C8E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Nuclear relocation of Kss1 contributes to the specificity of the mating response.
Journal
Scientific reports
Author(s)
Pelet S.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
06/03/2017
Peer-reviewed
Oui
Volume
7
Pages
43636
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Mitogen Activated Protein Kinases (MAPK) play a central role in transducing extra-cellular signals into defined biological responses. These enzymes, conserved in all eukaryotes, exert their function via the phosphorylation of numerous substrates located throughout the cell and by inducing a complex transcriptional program. The partitioning of their activity between the cytoplasm and the nucleus is thus central to their function. Budding yeast serves as a powerful system to understand the regulation of these fundamental biological phenomena. Under vegetative growth, the MAPK Kss1 is enriched in the nucleus of the cells. Stimulation with mating pheromone results in a rapid relocation of the protein in the cytoplasm. Activity of either Fus3 or Kss1 in the mating pathway is sufficient to drive this change in location by disassembling the complex formed between Kss1, Ste12 and Dig1. Artificial enrichment of the MAPK Kss1 in the nucleus in presence of mating pheromone alters the transcriptional response of the cells and induces a cell-cycle arrest in absence of Fus3 and Far1.
Keywords
Alleles, Cell Cycle Checkpoints, Cell Nucleus/metabolism, Enzyme Activation, Fungal Proteins/metabolism, Gene Expression Regulation, Fungal, Mitogen-Activated Protein Kinases/genetics, Mitogen-Activated Protein Kinases/metabolism, Models, Biological, Protein Binding, Protein Transport, Signal Transduction
Pubmed
Web of science
Open Access
Yes
Create date
14/03/2017 11:56
Last modification date
20/08/2019 16:34
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