Effects of hyperinsulinemia and hyperglycemia on lactate release and local blood flow in subcutaneous adipose tissue of healthy humans

Details

Serval ID
serval:BIB_BF5A2027376A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effects of hyperinsulinemia and hyperglycemia on lactate release and local blood flow in subcutaneous adipose tissue of healthy humans
Journal
Journal of Clinical Endocrinology and Metabolism
Author(s)
Henry  S., Schneiter  P., Jequier  E., Tappy  L.
ISSN
0021-972X (Print)
Publication state
Published
Issued date
08/1996
Volume
81
Number
8
Pages
2891-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Abstract
To determine the effects of hyperglycemia and hyperinsulinemia on lactate production by adipocytes, healthy volunteers were studied during three experimental protocols. In protocol 1, the changes in interstitial lactate concentrations were measured by microdialysis (sc tissue) after oral glucose administration. The plasma lactate concentration increased by 39.4 +/- 6.0%, and the dialysate lactate concentration increased by 117.9 +/- 16.3%. In protocol 2, a 2.5-h hyperinsulinemic euglycemic clamp and somatostatin infusion were performed. The plasma and dialysate lactate concentrations increased by 27.1 +/- 5.5% and 146.8 +/- 44.5%, respectively. In addition, [U-13C]glucose was infused through the probe, and dialysate lactate was enriched in 13C at 2.5 +/- 0.3 molar percent excess basally and at 3.4 +/- 0.3 molar percent excess during the clamp (P < 0.05 vs. basal). [13C]Urea was also infused through the probe, and the outflow to inflow ratio of [13C]urea was used as an index of local blood flow. It decreased by 10.2 +/- 3.6% (P < 0.001) at the end of the hyperinsulinemic euglycemic clamp, indicating an increase in blood flow. In protocol 3, a hyperglycemic clamp (10.0 mmol/L) at the basal insulin concentration was performed. It increased the dialysate lactate concentration by 43.5 +/- 15.9% and did not alter the plasma lactate concentration or local blood flow. It is concluded that hyperinsulinemia and, to a lesser extent, hyperglycemia stimulate glucose conversion into lactate in adipocytes. Hyperinsulinemia, but not hyperglycemia, also increases adipose tissue blood flow.
Keywords
Adipose Tissue/*blood supply/*metabolism Administration, Oral Adult Drinking Glucose/pharmacology Glucose Clamp Technique Humans Hyperglycemia/metabolism/*physiopathology Hyperinsulinism/metabolism/*physiopathology Insulin/blood Lactic Acid/*metabolism Male Osmolar Concentration Reference Values Regional Blood Flow/drug effects
Pubmed
Web of science
Create date
24/01/2008 13:36
Last modification date
20/08/2019 15:33
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