Selective neurofilament (SMI-32, FNP-7 and N200) expression in subpopulations of layer V pyramidal neurons in vivo and in vitro

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Serval ID
serval:BIB_BF4572F61351
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Selective neurofilament (SMI-32, FNP-7 and N200) expression in subpopulations of layer V pyramidal neurons in vivo and in vitro
Journal
Cerebral Cortex
Author(s)
Voelker  C. C., Garin  N., Taylor  J. S., Gahwiler  B. H., Hornung  J. P., Molnar  Z.
ISSN
1047-3211 (Print)
Publication state
Published
Issued date
11/2004
Volume
14
Number
11
Pages
1276-86
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
There are two main types of layer V pyramidal neurons in rat cortex. Type I neurons have tufted apical dendrites extending into layer I, produce bursts of action potentials and project to subcortical targets (spinal cord, superior colliculus and pontine nuclei). Type II neurons have apical dendrites, which arborize in layers II-IV, do not produce bursts of action potentials and project to ipsilateral and contralateral cortex. The specific expression of different genes and proteins in these two distinct layer V neurons is unknown. To distinguish between distinct subpopulations, fluorescent microspheres were injected into subcortical targets (labeling type I neurons) or primary somatosensory cortex (labeling type II neurons) of adult rats. After transport, cortical sections were processed for immunohistochemistry using various antibodies. This study demonstrated that antigens recognized by SMI-32, N200 and FNP-7 antibodies were only expressed in subcortical (type I)--but not in contralateral (type II)--projecting neurons. NR1, NR2a/b, PLCbeta1, BDNF, NGF and TrkB antigens were highly expressed in all neuronal subpopulations examined. Organotypic culture experiments demonstrated that the development of neurofilament expression and laminar specificity does not depend on the presence of the subcortical targets. This study suggests specific markers for the subcortical projecting layer V neuron subpopulations.
Keywords
Animals Gene Expression Regulation/physiology Neurofilament Proteins/analysis/*biosynthesis Pyramidal Cells/chemistry/cytology/*metabolism Rats Rats, Wistar
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:22
Last modification date
25/09/2019 7:10
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