The complete and fully assembled genome sequence of Aeromonas salmonicida subsp. pectinolytica and its comparative analysis with other Aeromonas species: investigation of the mobilome in environmental and pathogenic strains.

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State: Public
Version: Final published version
Serval ID
serval:BIB_BF2A2E62E4A5
Type
Article: article from journal or magazin.
Collection
Publications
Title
The complete and fully assembled genome sequence of Aeromonas salmonicida subsp. pectinolytica and its comparative analysis with other Aeromonas species: investigation of the mobilome in environmental and pathogenic strains.
Journal
BMC genomics
Author(s)
Pfeiffer F., Zamora-Lagos M.A., Blettinger M., Yeroslaviz A., Dahl A., Gruber S., Habermann B.H.
ISSN
1471-2164 (Electronic)
ISSN-L
1471-2164
Publication state
Published
Issued date
05/01/2018
Peer-reviewed
Oui
Volume
19
Number
1
Pages
20
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Due to the predominant usage of short-read sequencing to date, most bacterial genome sequences reported in the last years remain at the draft level. This precludes certain types of analyses, such as the in-depth analysis of genome plasticity.
Here we report the finalized genome sequence of the environmental strain Aeromonas salmonicida subsp. pectinolytica 34mel, for which only a draft genome with 253 contigs is currently available. Successful completion of the transposon-rich genome critically depended on the PacBio long read sequencing technology. Using finalized genome sequences of A. salmonicida subsp. pectinolytica and other Aeromonads, we report the detailed analysis of the transposon composition of these bacterial species. Mobilome evolution is exemplified by a complex transposon, which has shifted from pathogenicity-related to environmental-related gene content in A. salmonicida subsp. pectinolytica 34mel.
Obtaining the complete, circular genome of A. salmonicida subsp. pectinolytica allowed us to perform an in-depth analysis of its mobilome. We demonstrate the mobilome-dependent evolution of this strain's genetic profile from pathogenic to environmental.
Keywords
Aeromonas/genetics, Aeromonas salmonicida/genetics, Aeromonas salmonicida/isolation & purification, Aeromonas salmonicida/pathogenicity, DNA Transposable Elements, Environmental Microbiology, Genes, Bacterial, Genome, Bacterial, Genomics, High-Throughput Nucleotide Sequencing, Interspersed Repetitive Sequences, Aeromonas, Complete circular genome, Environmental, High-precision sequencing, Long-read sequencing, Mobilome, Mobilome-dependent evolution, PacBio, Pathogenic, Transposon
Pubmed
Web of science
Open Access
Yes
Create date
22/01/2018 12:31
Last modification date
20/08/2019 16:33
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