Ligand-induced assembly and activation of the gamma interferon receptor in intact cells.

Details

Serval ID
serval:BIB_BF1A7E275F66
Type
Article: article from journal or magazin.
Collection
Publications
Title
Ligand-induced assembly and activation of the gamma interferon receptor in intact cells.
Journal
Molecular and Cellular Biology
Author(s)
Bach E.A., Tanner J.W., Marsters S., Ashkenazi A., Aguet M., Shaw A.S., Schreiber R.D.
ISSN
0270-7306 (Print)
ISSN-L
0270-7306
Publication state
Published
Issued date
1996
Volume
16
Number
6
Pages
3214-3221
Language
english
Abstract
Functionally active gamma interferon (IFN-gamma) receptors consist of an alpha subunit required for ligand binding and signal transduction and a beta subunit required primarily for signaling. Although the receptor alpha chain has been well characterized, little is known about the specific role of the receptor beta chain in IFN-gamma signaling. Expression of the wild-type human IFN-gamma receptor beta chain in murine L cells that stably express the human IFN-gamma receptor alpha chain (L.hgR) produced a murine cell line (L.hgR.myc beta) that responded to human IFN-gamma. Mutagenesis of the receptor beta-chain intracellular domain revealed that only two closely spaced, membrane-proximal sequences (P263PSIP267 and I270EEYL274) are required for function. Coprecipitation studies showed that these sequences are necessary for the specific and constitutive association of the receptor beta chain with the JAK-2 tyrosine kinase. These experiments also revealed that the IFN-gamma receptor alpha and beta chains are not preassociated on the surface of unstimulated cells but rather are induced to associate in an IFN-gamma-dependent fashion. A chimeric protein in which the intracellular domain of the beta chain was replaced by JAK-2 complemented human IFN-gamma signaling and biologic responsiveness in L.hgR. In contrast, a c-src-containing beta-chain chimera did not. These results indicate that the sole obligate role of the IFN-gamma receptor beta chain in signaling is to recruit JAK-2 into the ligand-assembled receptor complex.
Keywords
Amino Acid Sequence, Animals, Antigens, CD/drug effects, Antigens, CD/genetics, Binding Sites/genetics, Humans, Interferon-gamma/pharmacology, Janus Kinase 2, L Cells (Cell Line), Ligands, Mice, Models, Biological, Molecular Sequence Data, Mutagenesis, Site-Directed, Protein Conformation, Protein-Tyrosine Kinases/metabolism, Proto-Oncogene Proteins, Receptors, Interferon/drug effects, Receptors, Interferon/genetics, Recombinant Proteins/chemistry, Recombinant Proteins/genetics, Signal Transduction/immunology
Pubmed
Web of science
Create date
28/01/2008 11:36
Last modification date
20/08/2019 15:33
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