Cryo-electron microscopy of viruses

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State: Serval
Version: Final published version
Secondary document(s)
Sous embargo indéterminé.
State: Serval
Version: Supplementary document
Serval ID
serval:BIB_BEC796503260
Type
Article: article from journal or magazin.
Collection
Publications
Title
Cryo-electron microscopy of viruses
Journal
Nature
Author(s)
Adrian  M., Dubochet  J., Lepault  J., McDowall  A. W.
ISSN
0028-0836 (Print)
Publication state
Published
Issued date
03/1984
Peer-reviewed
Oui
Volume
308
Number
5954
Pages
32-6
Language
english
Notes
Journal Article --- Old month value: Mar 1-7
Abstract
Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional electron microscopy. Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare favourably with any other electron microscopical method.
Keywords
Adenoviruses, Human/ultrastructure Bacteriophage lambda/ultrastructure Escherichia coli/ultrastructure Freezing Microscopy, Electron/methods Semliki forest virus/ultrastructure T-Phages/ultrastructure Vesicular stomatitis-Indiana virus/ultrastructure Viruses/*ultrastructure
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 11:25
Last modification date
09/05/2019 0:37
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