Pom1 regulates the assembly of Cdr2-Mid1 cortical nodes for robust spatial control of cytokinesis.
Details
State: Public
Version: Final published version
Serval ID
serval:BIB_BE73EE8BE19D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Pom1 regulates the assembly of Cdr2-Mid1 cortical nodes for robust spatial control of cytokinesis.
Journal
Journal of Cell Biology
ISSN
1540-8140 (Electronic)
ISSN-L
0021-9525
Publication state
Published
Issued date
2014
Volume
206
Number
1
Pages
61-77
Language
english
Abstract
Proper division plane positioning is essential to achieve faithful DNA segregation and to control daughter cell size, positioning, or fate within tissues. In Schizosaccharomyces pombe, division plane positioning is controlled positively by export of the division plane positioning factor Mid1/anillin from the nucleus and negatively by the Pom1/DYRK (dual-specificity tyrosine-regulated kinase) gradients emanating from cell tips. Pom1 restricts to the cell middle cortical cytokinetic ring precursor nodes organized by the SAD-like kinase Cdr2 and Mid1/anillin through an unknown mechanism. In this study, we show that Pom1 modulates Cdr2 association with membranes by phosphorylation of a basic region cooperating with the lipid-binding KA-1 domain. Pom1 also inhibits Cdr2 interaction with Mid1, reducing its clustering ability, possibly by down-regulation of Cdr2 kinase activity. We propose that the dual regulation exerted by Pom1 on Cdr2 prevents Cdr2 assembly into stable nodes in the cell tip region where Pom1 concentration is high, which ensures proper positioning of cytokinetic ring precursors at the cell geometrical center and robust and accurate division plane positioning.
Keywords
Chromosome Segregation, Chromosomes, Fungal/metabolism, Cytokinesis, Hydrophobic and Hydrophilic Interactions, Phosphorylation, Protein Binding, Protein Kinases/physiology, Protein Multimerization, Protein Processing, Post-Translational, Protein Structure, Tertiary, Protein Transport, Protein-Serine-Threonine Kinases/chemistry, Protein-Serine-Threonine Kinases/metabolism, Schizosaccharomyces/cytology, Schizosaccharomyces/metabolism, Schizosaccharomyces pombe Proteins/chemistry, Schizosaccharomyces pombe Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
05/02/2015 10:37
Last modification date
20/08/2019 16:32