The effect of pH-neutral peritoneal dialysis fluids on adipokine secretion from cultured adipocytes

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Serval ID
serval:BIB_BDD65D2A0F8B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The effect of pH-neutral peritoneal dialysis fluids on adipokine secretion from cultured adipocytes
Journal
Nephrology, Dialysis, Transplantation
Author(s)
Teta  D., Maillard  M., Tedjani  A., Passlick-Deetjen  J., Burnier  M.
ISSN
0931-0509 (Print)
Publication state
Published
Issued date
03/2007
Volume
22
Number
3
Pages
862-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
BACKGROUND: Adipokines are a group of fat-secreted hormones and cytokines, including leptin and adiponectin, with important functions in humans. Peritoneal dialysis (PD) is associated with markedly raised plasma adipokines, suggesting increased production in this setting. We have shown that low pH down-regulates leptin production. The current study was designed to test if novel pH-neutral PD fluids may regulate leptin and adiponectin secretion in vitro. METHODS: We exposed 3T3-L1 adipocytes to a 50 : 50 mixture of dialysate and M199 containing 10% serum for upto 48 h. Dialysates were commercial PD fluids, i.e. conventional acidic, lactate-buffered solutions (PD-acid) and pH-neutral lactate-buffered (PD-Bal) or bicarbonate-buffered solutions (PD-Bic). Leptin and adiponectin concentrations in culture-cell media were measured by ELISA. RESULTS: Compared with PD-acid, PD-Bal and PD-Bic produced a 25 and 43% increase, respectively, in leptin secretion at 48 h (P < 0.05). In contrast, adiponectin secretion was not affected. High glucose PD fluids (4.25%) specifically inhibited leptin secretion vs 1.5% glucose, buffer-matched solutions (P < 0.05). However, differences in leptin secretion due to pH and type of buffer remained significant. In further experiments, the pH of test media were extensively varied without the presence of dialysates. Leptin secretion was shown to increase in a parallel to pH, whereas large changes in pH did not affect adiponectin secretion. CONCLUSION: The pH-neutral PD solutions specifically induce leptin, but not adiponectin secretion from 3T3-L1 adipocytes. PD-Bic produced a greater leptin stimulation than PD-Bal, but this difference was attributable to pH per se, rather than the type of buffer.
Keywords
Adipocytes/drug effects/*metabolism Adiponectin/analysis/*secretion Cells, Cultured Dialysis Solutions/*chemistry/*pharmacology Enzyme-Linked Immunosorbent Assay Humans Hydrogen-Ion Concentration Leptin/analysis/*secretion Omentum/pathology *Peritoneal Dialysis
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 14:01
Last modification date
01/10/2019 7:19
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