Three-stranded DNA structure; is this the secret of DNA homologous recognition?

Details

Serval ID
serval:BIB_BDBC643D5160
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Three-stranded DNA structure; is this the secret of DNA homologous recognition?
Journal
Molecular Microbiology
Author(s)
Stasiak A.
ISSN
0950-382X[print], 0950-382X[linking]
Publication state
Published
Issued date
11/1992
Volume
6
Number
22
Pages
3267-3276
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review
Publication Status: ppublish
Abstract
A novel type of triple-stranded DNA structure was proposed by several groups to play a crucial role in homologous recognition between single- and double-stranded DNA molecules. In this still putative structure a duplex DNA was proposed to co-ordinate a homologous single strand in its major groove side. In contrast to the well-characterized pyrimidine-purine-pyrimidine triplexes in which the two like strands are antiparallel and which are restricted to poly-pyrimidine-containing stretches, the homology-specific triplexes would have like strands in parallel orientation and would not be restricted to any particular sequence provided that there is a homology between interacting DNA molecules. For many years the stereo-chemical possibility of forming homology-dependent three- or four-stranded DNA structures during the pairing stage of recombination reactions was seriously considered in published papers. However, only recently has there been a marked increase in the number of papers that have directly tested the formation of triple-stranded DNA structures during the actual pairing stage of the recombination reaction. Unfortunately the results of these tests are not totally clear cut; while some laboratories presented experimental evidence consistent with the formation of triplexes, others studying the same or very similar systems offered alternative explanations. The aim of this review is to present the current state of the central question in the mechanism of homologous recombination, namely, what kind of DNA structure is responsible for DNA homologous recognition. Is it a novel triplex structure or just a classical duplex?
Keywords
DNA/chemistry, DNA/ultrastructure, DNA Nucleotidyltransferases/metabolism, Integrases, Models, Genetic, Models, Molecular, Nucleic Acid Conformation, Rec A Recombinases/metabolism, Recombinases, Recombination, Genetic, Sequence Homology, Nucleic Acid
Pubmed
Web of science
Create date
24/01/2008 10:36
Last modification date
20/08/2019 15:31
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