Baroreflex and atrial natriuretic factor concentration correlate with myocardial infarct size and predict early death in rabbits: implications for drug studies
Details
Serval ID
serval:BIB_BD8AC35FF1A2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Baroreflex and atrial natriuretic factor concentration correlate with myocardial infarct size and predict early death in rabbits: implications for drug studies
Journal
Journal of Cardiovascular Pharmacology
ISSN
0160-2446 (Print)
Publication state
Published
Issued date
09/1991
Volume
18
Number
3
Pages
361-8
Notes
Journal Article --- Old month value: Sep
Abstract
The severity of myocardial infarction (MI) and its functional consequences are difficult to assess in small animals. We searched for criteria to achieve such an assessment in rabbits 1 week after MI. Thirteen large mongrel rabbits (3-4 kg) were anesthetized with pentobarbitone for ligating a branch of the circumflex coronary artery and 7 rabbits were subject to a sham operation without ligation. All sham-operated rabbits and 12 MI animals survived for 1 week, when blood was obtained for biochemical analyses and the baroreflex was tested. Six animals survived to the third week (survivors) and six died earlier (nonsurvivors). The MI size, measured immediately after death, was 42 +/- 3% of the left ventricular mass in nonsurvivors and 20 +/- 7% in survivors. The plasma atrial natriuretic factor (ANF) concentration was correlated linearly with MI size (r = 0.77) over the whole range of infarct sizes and, like the MI size itself, was associated with the risk of early death (critical limit: 80 pM). Plasma renin activity and catecholamines yielded less prognostic information. The baroreflex control of the heart rate (tested using phenylephrine and nitroprusside) of nonsurvivors was severely impaired and the slopes correlated with MI size (r = 0.90 for phenylephrine and r = 0.67 for nitroprusside). The plasma ANF concentration and the baroreflex both accurately reflected MI size and also correctly classified 11/12 rabbits into survivors and nonsurvivors. An ANF- and baroreflex-based stratification of animals for future studies on therapeutic interventions after MI will reduce the number of animals required by at least 65%, making such studies far more feasible than in the past.
Keywords
Animals
Atrial Natriuretic Factor/*metabolism
Blood Pressure/drug effects
Coronary Vessels/physiology
Female
Heart Rate/drug effects
Male
Myocardial Infarction/*pathology
Nitroprusside/diagnostic use
Norepinephrine/blood
Phenylephrine/diagnostic use
Pressoreceptors/*drug effects
Rabbits
Radioimmunoassay
Reflex/*drug effects
Renin/blood
Pubmed
Web of science
Create date
05/03/2008 16:40
Last modification date
20/08/2019 15:31