06: Visualization of hepatitis E virus RNA and proteins in the human liver

Details

Serval ID
serval:BIB_BD692F0177A7
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Abstract (Abstract): shot summary in a article that contain essentials elements presented during a scientific conference, lecture or from a poster.
Collection
Publications
Title
06: Visualization of hepatitis E virus RNA and proteins in the human liver
Title of the conference
Swiss Pathology Days
Author(s)
Lenggenhager D, Gouttenoire J., Malehmir M., Bawohl M., Kreutzer S., Semela D., Neuweiler J., Hürlimann S., Aepli P., Fraga M., Sahli R., Terracciano L., Rubbia-Brandt L., Müllhaupt B., Sempoux C., Moradpour D., Weber A.
Working group(s)
Honcharova, -Biletska, H.
Address
Thun, Switzerland, November 10-12, 2017
ISSN
0172-8113
1432-1963
ISSN-L
1432-1963
Publication state
Published
Issued date
20/10/2017
Volume
38
Number
6
Series
Der Pathologe
Pages
571-572
Language
english
Abstract
Background: Although hepatitis E constitutes a substantial disease burden
worldwide, surprisingly little is known about the localization of hepatitis E Virus (HEV) in the human liver. We therefore aimed to visualize HEV
RNA and proteins in situ.
Methods: Twelve antibodies against HEV open reading frame (ORF) 1–3
proteins for immunohistochemistry (IHC) and two probes for in situ
hybridization (ISH) were tested on formalin-fixed, paraffin-embedded
(FFPE) Huh-7 cells transfected with HEV ORF1-3 Expression vectors.
IHC (and partly ISH) were then applied to Hep293TT cells replicating infectious
HEV and liver specimens from patients with hepatitis E (n=20)
and controls (n=134).
Results: Whereas ORF1-3 proteins were all detectable in HEV protein expressing
cells, only ORF2 and 3 proteins were traceable in cells replicating
infectious HEV. In liver specimens from patients with hepatitis E, only the
ORF2encoded capsid protein was unequivocally detectable. IHC for ORF2
protein revealed a patchy expression in individual or grouped hepatocytes,
generally stronger in cases of chronic compared to acute hepatitis. Besides
cytoplasmic and canalicular, ORF2 protein also displayed a hitherto not
described nuclear localization. Furthermore, positivity for ORF2 protein
in defined areas correlated with HEV RNA detection by ISH. IHC was specific
and comparably sensitive as PCR for HEV RNA.
Conclusions: In livers from patients with hepatitis E, the ORF2 protein can
be visualized reliably by IHC, allowing sensitive and specific detection of
HEV in FFPE samples. Therefore, HEV ORF2 IHC can be used as a rapid,
handy and not expensive ancillary tool in histopathologic diagnostics of
HEV. In addition, its variable subcellular distribution in individual hepatocytes
of the same liver suggests an interaction with nuclear components
and thus a redistribution of ORF2 protein during infection.
Create date
13/11/2017 15:08
Last modification date
20/08/2019 16:31
Usage data