Characterization of immortalized human islet stromal cells reveals a MSC-like profile with pancreatic features.

Details

Serval ID
serval:BIB_BD54883E8A85
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Characterization of immortalized human islet stromal cells reveals a MSC-like profile with pancreatic features.
Journal
Stem cell research & therapy
Author(s)
Villard O., Armanet M., Couderc G., Bony C., Moreaux J., Noël D., DeVos J., Klein B., Veyrune J.L., Wojtusciszyn A.
ISSN
1757-6512 (Electronic)
ISSN-L
1757-6512
Publication state
Published
Issued date
17/04/2020
Peer-reviewed
Oui
Volume
11
Number
1
Pages
158
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Mesenchymal stromal cells (MSCs) represent an interesting tool to improve pancreatic islet transplantation. They have immunomodulatory properties and secrete supportive proteins. However, the functional properties of MSCs vary according to many factors such as donor characteristics, tissue origin, or isolation methods. To counteract this heterogeneity, we aimed to immortalize and characterize adherent cells derived from human pancreatic islets (hISCs), using phenotypic, transcriptomic, and functional analysis.
Adherent cells derived from human islets in culture were infected with a hTERT retrovirus vector and then characterized by microarray hybridization, flow cytometry analysis, and immunofluorescence assays. Osteogenic, adipogenic, and chondrogenic differentiation as well as PBMC proliferation suppression assays were used to compare the functional abilities of hISCs and MSCs. Extracellular matrix (ECM) gene expression profile analysis was performed using the SAM (Significance Analysis of Microarrays) software, and protein expression was confirmed by western blotting.
hISCs kept an unlimited proliferative potential. They exhibited several properties of MSCs such as CD73, CD90, and CD105 expression and differentiation capacity. From a functional point of view, hISCs inhibited the proliferation of activated peripheral blood mononuclear cells. The transcriptomic profile of hISCs highly clusterized with bone marrow (BM)-MSCs and revealed a differential enrichment of genes involved in the organization of the ECM. Indeed, the expression and secretion profiles of ECM proteins including collagens I, IV, and VI, fibronectin, and laminins, known to be expressed in abundance around and within the islets, were different between hISCs and BM-MSCs.
We generated a new human cell line from pancreatic islets, with MSCs properties and retaining some pancreatic specificities related to the production of ECM proteins. hISCs appear as a very promising tool in islet transplantation by their availability (as a source of inexhaustible source of cells) and ability to secrete a supportive "pancreatic" microenvironment.
Keywords
Cell therapy, Conditioned medium, Extracellular matrix, Mesenchymal stromal cells, Pancreatic islets
Pubmed
Open Access
Yes
Create date
25/04/2020 19:44
Last modification date
18/09/2021 6:38
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