Development of a real-time PCR for the specific detection of Waddlia chondrophila in clinical samples.

Details

Ressource 1Download: serval:BIB_BD348AF19844.P001 (113.44 [Ko])
State: Public
Version: author
License: Not specified
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Serval ID
serval:BIB_BD348AF19844
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Development of a real-time PCR for the specific detection of Waddlia chondrophila in clinical samples.
Journal
European Journal of Clinical Microbiology and Infectious Diseases
Author(s)
Goy G., Croxatto A., Posfay-Barbe K.M., Gervaix A., Greub G.
ISSN
1435-4373 (Electronic)
ISSN-L
0934-9723
Publication state
Published
Issued date
2009
Volume
28
Number
12
Pages
1483-1486
Language
english
Notes
Publication types: Case Reports ; Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Waddlia chondrophila is considered as an emerging human pathogen likely involved in miscarriage and lower respiratory tract infections. Given the low sensitivity of cell culture to recover such an obligate intracellular bacteria, molecular-based diagnostic approaches are warranted. We thus developed a real-time PCR that amplifies Waddlia chondrophila DNA. Specific primers and probe were selected to target the 16S rRNA gene. The PCR specifically amplified W. chondrophila but did not amplify other related-bacteria such as Parachlamydia acanthamoebae, Simkania negevensis and Chlamydia pneumoniae. The PCR exhibited a good intra-run and inter-run reproducibility and a sensitivity of less than ten copies of the positive control. This real-time PCR was then applied to 32 nasopharyngeal aspirates taken from children with bronchiolitis not due to respiratory syncytial virus (RSV). Three samples revealed to be Waddlia positive, suggesting a possible role of this Chlamydia-related bacteria in this setting.
Keywords
Bacteriological Techniques/methods, Child, Chlamydiales/genetics, Chlamydiales/isolation & purification, DNA Primers/genetics, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Female, Gram-Negative Bacterial Infections/diagnosis, Gram-Negative Bacterial Infections/microbiology, Humans, Infant, Infant, Newborn, Nasopharynx/microbiology, Polymerase Chain Reaction/methods, RNA, Ribosomal, 16S/genetics, Reproducibility of Results, Sensitivity and Specificity
Pubmed
Web of science
Open Access
Yes
Create date
24/11/2009 17:32
Last modification date
01/10/2019 7:19
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