Evaluation of the RAPIDEC® CARBA NP and β-CARBA® tests for rapid detection of Carbapenemase-producing Enterobacteriaceae.

Details

Serval ID
serval:BIB_BCE960C6906E
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Evaluation of the RAPIDEC® CARBA NP and β-CARBA® tests for rapid detection of Carbapenemase-producing Enterobacteriaceae.
Journal
Diagnostic microbiology and infectious disease
Author(s)
Mancini S., Kieffer N., Poirel L., Nordmann P.
ISSN
1879-0070 (Electronic)
ISSN-L
0732-8893
Publication state
Published
Issued date
08/2017
Peer-reviewed
Oui
Volume
88
Number
4
Pages
293-297
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The analytical performances of the RAPIDEC CARBA NP® (bioMérieux) and the β-CARBA® (Bio-Rad) tests were evaluated for the rapid detection of any known type of carbapenemases in Enterobacteriaceae. An international collection of 149 enterobacterial isolates comprising 111 Carbapenemase-Producing Enterobacteriaceae (CPE) and 38 non-carbapenemase producers was used. CPE included 32 carbapenemase producers of class A (18 KPC-2, 1 FRI-1, 5 SME and 8 IMI), 33 of class B (13 NDM, 11 VIM and 9 IMP) and 46 of class D (15 OXA-48, 14 OXA-181, 10 OXA-204, 3 OXA-232 and 4 OXA-162). The RAPIDEC CARBA NP® and the β-CARBA® tests were performed in strict accordance to the manufacturer's instructions and results were read within 2 h and 30 min, respectively. RAPIDEC CARBA NP® detected 104/111 CPE isolates compared to 72/111 for the β-CARBA® test. The overall sensitivity and specificity were 93.7% and 100%, respectively, for the RAPIDEC CARBA NP® test, and 64.9% and 90%, respectively, for the β-CARBA® test. The β-CARBA® test failed to detect all the non-KPC class A carbapenemases (14/14) and most (24/31) of the OXA-48-like producers (OXA-162, OXA-181, OXA-204 and OXA-232), and detected 1/1 OXA-163 and 1/1 OXA-405 as carbapenemase producers whereas these enzymes are rather defined as non carbapenemases. RAPIDEC CARBA NP® test exhibited better performances than those of the β-CARBA® test and confirmed to be a reliable tool for the detection of CPE, especially for OXA-48-like producers.

Keywords
Bacteriological Techniques/methods, Carbapenem-Resistant Enterobacteriaceae/metabolism, Enterobacteriaceae/metabolism, Microbial Sensitivity Tests/methods, Sensitivity and Specificity, beta-Lactamases/metabolism, RAPIDEC CARBA NP® test, Rapid carbapenemase detection, β-CARBA® test
Pubmed
Web of science
Create date
30/05/2017 16:14
Last modification date
20/08/2019 15:31
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