Differential increase in the cholinergic properties of cultured chick sympathetic neurons by forskolin
Details
Serval ID
serval:BIB_BC1853B238CD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differential increase in the cholinergic properties of cultured chick sympathetic neurons by forskolin
Journal
Developmental Biology
ISSN
0012-1606 (Print)
Publication state
Published
Issued date
07/1990
Volume
140
Number
1
Pages
53-6
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Abstract
Sympathetic neurons from the chick superior cervical ganglion (SCG) in culture synthesize and accumulate catecholamine (CA), and to a lesser extent, acetylcholine (AcCho). The noradrenergic and cholinergic properties of these cells can be differentially modulated by various external factors such as neuronal density, chick eye extract, liver-conditioned-medium, and inosine. We report here that forskolin, an activator of adenylate cyclase, selectively promotes AcCho, but not CA production in the chick SCG neurons. This effect is rapid since an increase in the AcCho content is already observed 60 min after the addition of forskolin to 2-week-old cultures. Rapid gene induction or protein phosphorylation via a cAMP-dependent pathway may thus be involved in the differential regulation of acetylcholine metabolism in cultured chick sympathetic neurons.
Keywords
8-Bromo Cyclic Adenosine Monophosphate/pharmacology
Acetylcholine/*biosynthesis
Animals
Bucladesine/pharmacology
Butyrates/pharmacology
Catecholamines/*biosynthesis
Cells, Cultured
Chick Embryo
Dose-Response Relationship, Drug
Forskolin/analogs & derivatives/*pharmacology
Ganglia, Sympathetic/*drug effects/metabolism
Pubmed
Web of science
Create date
28/01/2008 8:44
Last modification date
20/08/2019 15:30