Normal responsiveness of CTLA-4-deficient anti-viral cytotoxic T cells

Details

Serval ID
serval:BIB_BBFDC6D20CEB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Normal responsiveness of CTLA-4-deficient anti-viral cytotoxic T cells
Journal
Journal of Immunology
Author(s)
Bachmann  M. F., Waterhouse  P., Speiser  D. E., McKall-Faienza  K., Mak  T. W., Ohashi  P. S.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
01/1998
Volume
160
Number
1
Pages
95-100
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 1
Abstract
CTLA-4 has been proposed to negatively regulate immune responses, and mice deficient for CTLA-4 expression succumb to a lymphoproliferative disorder within a few weeks after birth. This study assessed the responsiveness of CTLA-4-deficient T cells expressing a class I-restricted TCR specific for lymphocytic choriomeningitis virus (LCMV). The kinetics of T cell proliferation were studied in vitro after stimulation of T cells with full and partial T cell agonists. No gross abnormalities in CTLA-4-deficient T cells could be detected. Using adoptive transfer experiments, T cell responses were also measured in vivo after infection with LCMV. Low dose infection with LCMV leads to strong expansion of specific T cells followed by a reduction in T cells that parallels the elimination of Ag. The kinetics of T cell expansion and elimination after low dose LCMV infection were not affected by the absence of CTLA-4. High dose infection of mice with LCMV leads to a transient expansion of T cells followed by T cell exhaustion, where all specific T cells are eliminated. T cell exhaustion also occurred in the absence of CTLA-4. Thus, surprisingly, the absence of CTLA-4 did not interfere with T cell activation, down-regulation of ongoing T cell responses after the elimination of Ag, or the exhaustion of T cell responses in the presence of excessive amounts of Ag.
Keywords
Amino Acid Sequence Animals Antigens, CD Antigens, Differentiation/*physiology CD8-Positive T-Lymphocytes/*immunology *Immunoconjugates Lymphocytic Choriomeningitis/immunology Mice Mice, Knockout Mice, Transgenic Peptides/immunology T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Create date
28/01/2008 11:32
Last modification date
20/08/2019 15:30
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