Localization of carboxyl ester lipase in human pituitary gland and pituitary adenomas.

Details

Serval ID
serval:BIB_BB9D8D607D95
Type
Article: article from journal or magazin.
Collection
Publications
Title
Localization of carboxyl ester lipase in human pituitary gland and pituitary adenomas.
Journal
Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Author(s)
La Rosa S., Vigetti D., Placidi C., Finzi G., Uccella S., Clerici M., Bartolini B., Carnevali I., Losa M., Capella C.
ISSN
1551-5044 (Electronic)
ISSN-L
0022-1554
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
58
Number
10
Pages
881-889
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Carboxyl ester lipase (CEL) is an enzyme that hydrolyzes a wide variety of lipid substrates, including ceramides, which are known to show inhibitory regulation of pituitary hormone secretion in experimental models. Because no studies on CEL expression in human pituitary and pituitary adenomas have been reported in the literature, we investigated CEL expression in 10 normal pituitary glands and 86 well-characterized pituitary adenomas [12 FSH/LH cell, 17 α-subunit/null cell, 6 TSH cell, 21 ACTH cell, 11 prolactin (PRL) cell, and 19 GH cell adenomas] using IHC, immunoelectron microscopy, Western blotting, and quantitative RT-PCR. In normal adenohypophysis, CEL was localized in GH, ACTH, and TSH cells. In adenomas, it was mainly found in functioning GH, ACTH, and TSH tumors, whereas its expression was poor in the corresponding silent adenomas and was lacking in FSH/LH cell, null cell, and PRL cell adenomas. Ultrastructurally, CEL was localized in secretory granules close to their membranes. This is the first study demonstrating CEL expression in normal human pituitary glands and in functioning GH, ACTH, and TSH adenomas. Considering that CEL hydrolyzes ceramides, inactivating their inhibitory function on pituitary hormone secretion, our findings suggest a possible role of CEL in the regulation of hormone secretion in both normal and adenomatous pituitary cells.
Keywords
Adenoma/enzymology, Cell Line, Cell Line, Tumor, Humans, Immunohistochemistry, Lipase/biosynthesis, Microscopy, Immunoelectron, Pituitary Gland/enzymology, Pituitary Neoplasms/enzymology, Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Create date
06/09/2016 13:53
Last modification date
20/08/2019 15:29
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