Parallel Reaction Monitoring-Based Quantification of Cannabinoids in Whole Blood.

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State: Public
Version: Author's accepted manuscript
License: Not specified
Serval ID
serval:BIB_BAF7EEFAE3BD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Parallel Reaction Monitoring-Based Quantification of Cannabinoids in Whole Blood.
Journal
Journal of analytical toxicology
Author(s)
Joye T., Widmer C., Favrat B., Augsburger M., Thomas A.
ISSN
1945-2403 (Electronic)
ISSN-L
0146-4760
Publication state
Published
Issued date
31/07/2020
Peer-reviewed
Oui
Volume
44
Number
6
Pages
541-548
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Cannabis is the most consumed drug of abuse, making it the primary target for identification and quantification in human whole blood regarding forensic and clinical toxicology analyses. Among biological matrices, blood is the reference for toxicological interpretation. A highly sensitive and selective liquid chromatography (LC) hyphenated with high-resolution mass spectrometry (HRMS) was developed for the quantification of Δ9-tetrahydrocannabinol (THC), 11-hydroxytetrahydrocannabinol (THC-OH), 11-nor-9-carboxy-tetrahydrocannabinol (THC-COOH) and cannabidiol (CBD). Those cannabinoids were extracted from 1 mL of whole blood by a simple liquid-liquid extraction (LLE) in acidic conditions. HRMS was performed on an Orbitrap-based instrument using its trapping capabilities and increased selectivity for parallel reaction monitoring (PRM) quantification in positive polarity with a negative polarity switching for THC-OH and THC-COOH. Although selected reaction monitoring (SRM) and PRM-targeted methods have similar performance in terms of linearity, dynamic range, precision and repeatability, Orbitrap-based PRM provides a higher specificity due to the use of high-resolution mode separating background ions from the targeted molecules. The method was fully validated according to guidelines set forth by the "Société Française des Sciences et des Techniques Pharmaceutiques" (SFSTP). Trueness was measured below 107% for all tested concentrations. Repeatability and intermediate precision were found to be lower than 12% while the assay was found to be linear in the concentration range of 0.4-20 ng/mL for THC, THC-OH and CBD and of 2-100 ng/mL for THC-COOH. Recovery (RE) and matrix effect (ME) ranged from 70.6 to 102.5% and from -40 to 6.6%, respectively. The validated method provides an efficient procedure for the simultaneous and rapid quantification of cannabinoids in PRM mode providing an alternative over classical SRM.
Pubmed
Create date
08/01/2020 9:48
Last modification date
19/09/2020 6:21
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