Integrative study of diet-induced mouse models of NAFLD identifies PPARα as a sexually dimorphic drug target.

Details

Serval ID
serval:BIB_BAA595ED824C
Type
Article: article from journal or magazin.
Publication sub-type
Minutes: analyse of a published work.
Collection
Publications
Institution
Title
Integrative study of diet-induced mouse models of NAFLD identifies PPARα as a sexually dimorphic drug target.
Journal
Gut
Author(s)
Smati S., Polizzi A., Fougerat A., Ellero-Simatos S., Blum Y., Lippi Y., Régnier M., Laroyenne A., Huillet M., Arif M., Zhang C., Lasserre F., Marrot A., Al Saati T., Wan J., Sommer C., Naylies C., Batut A., Lukowicz C., Fougeray T., Tramunt B., Dubot P., Smith L., Bertrand-Michel J., Hennuyer N., Pradere J.P., Staels B., Burcelin R., Lenfant F., Arnal J.F., Levade T., Gamet-Payrastre L., Lagarrigue S., Loiseau N., Lotersztajn S., Postic C., Wahli W., Bureau C., Guillaume M., Mardinoglu A., Montagner A., Gourdy P., Guillou H.
ISSN
1468-3288 (Electronic)
ISSN-L
0017-5749
Publication state
Published
Issued date
26/04/2021
Peer-reviewed
Oui
Language
english
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Abstract
We evaluated the influence of sex on the pathophysiology of non-alcoholic fatty liver disease (NAFLD). We investigated diet-induced phenotypic responses to define sex-specific regulation between healthy liver and NAFLD to identify influential pathways in different preclinical murine models and their relevance in humans.
Different models of diet-induced NAFLD (high-fat diet, choline-deficient high-fat diet, Western diet or Western diet supplemented with fructose and glucose in drinking water) were compared with a control diet in male and female mice. We performed metabolic phenotyping, including plasma biochemistry and liver histology, untargeted large-scale approaches (liver metabolome, lipidome and transcriptome), gene expression profiling and network analysis to identify sex-specific pathways in the mouse liver.
The different diets induced sex-specific responses that illustrated an increased susceptibility to NAFLD in male mice. The most severe lipid accumulation and inflammation/fibrosis occurred in males receiving the high-fat diet and Western diet, respectively. Sex-biased hepatic gene signatures were identified for these different dietary challenges. The peroxisome proliferator-activated receptor α (PPARα) co-expression network was identified as sexually dimorphic, and in vivo experiments in mice demonstrated that hepatocyte PPARα determines a sex-specific response to fasting and treatment with pemafibrate, a selective PPARα agonist. Liver molecular signatures in humans also provided evidence of sexually dimorphic gene expression profiles and the sex-specific co-expression network for PPARα.
These findings underscore the sex specificity of NAFLD pathophysiology in preclinical studies and identify PPARα as a pivotal, sexually dimorphic, pharmacological target.
NCT02390232.
Keywords
gene expression, lipid metabolism, liver metabolism, nonalcoholic steatohepatitis
Pubmed
Create date
11/05/2021 9:34
Last modification date
29/05/2021 6:32
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