Synthesis of multifunctional lipid-polymer conjugates: application to the elaboration of bright far-red fluorescent lipid probes dagger

Details

Serval ID
serval:BIB_BA3795EFDB5C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Synthesis of multifunctional lipid-polymer conjugates: application to the elaboration of bright far-red fluorescent lipid probes dagger
Journal
RSC Advances
Author(s)
Adjili S., Favier A., Massin J., Bretonnière Y., Lacour W., Lin Y.-C., Chatre E., Place C., Favard C., Muriaux D., Andraud C., Charreyre M.-T.
ISSN
2046-2069 (Electronic)
Publication state
Published
Issued date
03/2014
Peer-reviewed
Oui
Volume
4
Number
30
Pages
15569-15578
Language
french
Abstract
A new class of lipid-ended polymer conjugates presenting reactive sites regularly distributed along the polymer chain were synthesized using RAFT polymerization. The chosen modular approach enables preparation of different lipid families by tuning the nature of the phospholipid α-end, the molecular weight and the lateral functions of the polymer chain. The multiple activated ester functions of the conjugates can indeed be used for the efficient coupling of a great variety of amino-containing entities of interest. In this study, we elaborated original fluorescent lipid-polymer probes for optical microscopy by coupling along the chain a controlled number of chromophores emitting in the far-red where auto-fluorescence and light absorption by biological samples are limited. Water-soluble fluorescent lipid probes exhibiting an enhanced brightness were obtained. As a proof of concept, these probes were able to efficiently label the lipid bilayer of liposomes of various sizes. Such multifunctional lipid-ended polymers thus exhibit great potential to functionalize model and natural lipid assemblies.
Create date
22/04/2015 10:00
Last modification date
20/08/2019 15:28
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