Modulation of Secretion of Interleukin-6 in Brain-Derived Microvascular Endothelial Cells

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State: Public
Version: Final published version
License: All rights reserved
Serval ID
serval:BIB_BA0EB6920E9E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Modulation of Secretion of Interleukin-6 in Brain-Derived Microvascular Endothelial Cells
Journal
Endothelium
Author(s)
Juillerat-Jeanneret Lucienne, Fioroni Patricia, Leuenberger Philippe
ISSN
1062-3329
1029-2373
Publication state
Published
Issued date
01/1995
Peer-reviewed
Oui
Volume
3
Number
1
Pages
31-37
Language
english
Abstract
Vascular permeability is tightly controlled, in particular in the cerebral microvasculature, thus factors able to modulate permeability are of physiological importance. Interleukin-6 (IL-6) can be released by many cell types including mononuclear phagocytes, fibroblasts and endothelial cells of various origins, and is involved in a variety of cellular responses, including modulation of vascular permeability. IL-6 is synthesized as a precursor propeptide which is proteolytically processed to the active hormone, then degraded, by as yet unknown enzymatic pathways. Unstimulated brain-derived microvessels, primary cells and cell lines, all secreted bioactive IL-6. Cell lines secreted IL-6 exclusively as a 25 kD peptide. In order to understand mechanisms regulating the secretion of IL-6 in cerebral endothelium, levels of IL-6 were measured in cell culture supernatants of brain-derived endothelial cells exposed to dexamethasone, forskolin or dibutyryl cAMP and inhibitors of serine-proteases. In primary cells and in cell lines, dexamethasone and benzamidine (a non-covalent serine-protease inhibitor) significantly and time-dependently decreased, and forskolin, dibutyryl cAMP and 4-(2-aminoethyl)benzene-sulfonyl fluoride (a covalent serine-protease inhibitor) significantly and in a time-dependent fashion increased IL-6 levels in cell culture supernatants. These results demonstrate that IL-6 biological activity is regulated at several steps in cerebral endothelium
Keywords
Cell Biology, Physiology, General Medicine
Create date
30/01/2008 18:10
Last modification date
01/08/2020 5:19
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