Na(+)-K(+)-ATPase gene expression during in vitro development of rat fetal forebrain.

Details

Serval ID
serval:BIB_B9EB77E42D0A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Na(+)-K(+)-ATPase gene expression during in vitro development of rat fetal forebrain.
Journal
American Journal of Physiology. Cell Physiology
Author(s)
Corthésy-Theulaz I., Mérillat A.M., Honegger P., Rossier B.C.
ISSN
0363-6143
ISSN-L
0002-9513
Publication state
Published
Issued date
1990
Volume
258
Number
6 Pt 1
Pages
C1062-C1069
Language
english
Abstract
The existence of at least three isoforms of Na(+)-K(+)-ATPase in adult brain tissues [alpha 1, kidney type; alpha 2 [or alpha(+)]; alpha 3] suggests that these genes might be regulated in a cell-specific and time-dependent manner during development. We have studied this question in serum-free aggregating cell cultures of mechanically dissociated rat fetal telencephalon. At the protein level, the relative rate of synthesis of the pool of alpha 1-, alpha 2-, and alpha 3-subunits increased approximately twofold over 15 days of culture, leading to a marked increase in the immunochemical pool of alpha-subunits as measured by a panspecific polyclonal antibody. Concomitantly, Na(+)-K(+)-ATPase enzyme-specific activity increased three- (lower forebrain) to sixfold (upper forebrain). The transcripts of all three alpha-isoforms and beta-subunit were detected in vitro in similar proportion to the level observed in vivo. alpha 3-mRNA (3.7 kb) was more abundant than alpha 1 (3.7 kb) or alpha 2 (5.3 and 3.4 kb). Cytosine arabinoside (0.4 microM) and cholera toxin (0.1 microM) were used to selectively eliminate glial cells or neurons, respectively. It was found that alpha 2-mRNA is predominantly transcribed in glial cell cultures, whereas alpha 3- and beta 1-mRNA (2.7, 2.3, and 1.8 kb) are predominant in neuronal cultures.
Keywords
Animals, Blotting, Northern, Brain/enzymology, Cell Aggregation, Cells, Cultured, Cerebral Cortex/enzymology, Cholera Toxin/pharmacology, Cytarabine/pharmacology, Embryonic and Fetal Development/genetics, Gene Expression Regulation, Kidney/enzymology, Macromolecular Substances, Neuroglia/drug effects, Neurons/drug effects, RNA/genetics, RNA/isolation & purification, Rats, Sodium-Potassium-Exchanging ATPase/genetics, Sodium-Potassium-Exchanging ATPase/metabolism, Telencephalon/cytology, Telencephalon/embryology
Pubmed
Web of science
Create date
24/01/2008 13:00
Last modification date
20/08/2019 15:27
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