Activation of Cdc42 GTPase upon CRY2-Induced Cortical Recruitment Is Antagonized by GAPs in Fission Yeast.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_B9C88B605010
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Activation of Cdc42 GTPase upon CRY2-Induced Cortical Recruitment Is Antagonized by GAPs in Fission Yeast.
Journal
Cells
Author(s)
Lamas I., Weber N., Martin S.G.
ISSN
2073-4409 (Electronic)
ISSN-L
2073-4409
Publication state
Published
Issued date
12/09/2020
Peer-reviewed
Oui
Volume
9
Number
9
Pages
E2089
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The small GTPase Cdc42 is critical for cell polarization in eukaryotic cells. In rod-shaped fission yeast Schizosaccharomyces pombe cells, active GTP-bound Cdc42 promotes polarized growth at cell poles, while inactive Cdc42-GDP localizes ubiquitously also along cell sides. Zones of Cdc42 activity are maintained by positive feedback amplification involving the formation of a complex between Cdc42-GTP, the scaffold Scd2, and the guanine nucleotide exchange factor (GEF) Scd1, which promotes the activation of more Cdc42. Here, we use the CRY2-CIB1 optogenetic system to recruit and cluster a cytosolic Cdc42 variant at the plasma membrane and show that this leads to its moderate activation also on cell sides. Surprisingly, Scd2, which binds Cdc42-GTP, is still recruited to CRY2-Cdc42 clusters at cell sides in individual deletion of the GEFs Scd1 or Gef1. We show that activated Cdc42 clusters at cell sides are able to recruit Scd1, dependent on the scaffold Scd2. However, Cdc42 activity is not amplified by positive feedback and does not lead to morphogenetic changes, due to antagonistic activity of the GTPase activating protein Rga4. Thus, the cell architecture is robust to moderate activation of Cdc42 at cell sides.
Keywords
CRY2-CIBN, Cdc42, GTPase activating protein GAP, cell polarity, clustering, fission yeast Schizosaccharomyces pombe, optogenetics, pattern formation, positive feedback
Pubmed
Web of science
Open Access
Yes
Create date
19/09/2020 11:55
Last modification date
11/11/2020 7:10
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