A simple, robust and rapid approach to detect carbapenemases in Gram-negative isolates by MALDI-TOF mass spectrometry: validation with triple quadripole tandem mass spectrometry, microarray and PCR.

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State: Public
Version: Final published version
Serval ID
serval:BIB_B9BDBDD70CB6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A simple, robust and rapid approach to detect carbapenemases in Gram-negative isolates by MALDI-TOF mass spectrometry: validation with triple quadripole tandem mass spectrometry, microarray and PCR.
Journal
Clinical Microbiology and Infection
Author(s)
Vogne C., Prod'hom G., Jaton K., Decosterd L.A., Greub G.
ISSN
1469-0691 (Electronic)
ISSN-L
1198-743X
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
20
Number
12
Pages
O1106-O1112
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't; pdf : Original Article
Abstract
Carbapenemases should be accurately and rapidly detected, given their possible epidemiological spread and their impact on treatment options. Here, we developed a simple, easy and rapid matrix-assisted laser desorption ionization-time of flight (MALDI-TOF)-based assay to detect carbapenemases and compared this innovative test with four other diagnostic approaches on 47 clinical isolates. Tandem mass spectrometry (MS-MS) was also used to determine accurately the amount of antibiotic present in the supernatant after 1 h of incubation and both MALDI-TOF and MS-MS approaches exhibited a 100% sensitivity and a 100% specificity. By comparison, molecular genetic techniques (Check-MDR Carba PCR and Check-MDR CT103 microarray) showed a 90.5% sensitivity and a 100% specificity, as two strains of Aeromonas were not detected because their chromosomal carbapenemase is not targeted by probes used in both kits. Altogether, this innovative MALDI-TOF-based approach that uses a stable 10-μg disk of ertapenem was highly efficient in detecting carbapenemase, with a sensitivity higher than that of PCR and microarray.
Pubmed
Web of science
Open Access
Yes
Create date
17/02/2015 13:43
Last modification date
20/08/2019 15:27
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