Clinicopathologic and molecular genetic characterization of low-grade fibromyxoid sarcoma, and cloning of a novel FUS/CREB3L1 fusion gene

Details

Serval ID
serval:BIB_B9B9907BD917
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Clinicopathologic and molecular genetic characterization of low-grade fibromyxoid sarcoma, and cloning of a novel FUS/CREB3L1 fusion gene
Journal
Laboratory investigation; a journal of technical methods and pathology
Author(s)
Mertens  F., Fletcher  C. D., Antonescu  C. R., Coindre  J. M., Colecchia  M., Domanski  H. A., Downs-Kelly  E., Fisher  C., Goldblum  J. R., Guillou  L., Reid  R., Rosai  J., Sciot  R., Mandahl  N., Panagopoulos  I.
ISSN
0023-6837 (Print)
Publication state
Published
Issued date
2005
Volume
85
Number
3
Pages
408-415
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Abstract
Low-grade fibromyxoid sarcoma (LGFMS) is an indolent, late-metastasizing malignant soft-tissue tumor that is often mistaken for either more benign or more malignant tumor types. Cytogenetic analyses have identified a recurrent balanced translocation t(7;16) (q32-34;p11), later shown by molecular genetic approaches to result in a FUS/CREB3L2 fusion gene. Whereas preliminary studies suggest that this gene rearrangement is specific for LGFMS, its incidence in this tumor type and the possible existence of variant fusion genes have not yet been addressed. For this purpose, a series of potential LGFMS were obtained from nine different soft-tissue tumor centres and subjected to molecular analysis as well as careful histopathologic review. Reverse transcriptase-polymerase chain reaction analysis disclosed a FUS/CREB3L2 fusion transcript in 22 of the 23 (96%) cases that remained classified as LGFMS after the histologic re-evaluation and from which RNA of sufficient quality could be extracted, whereas none of the cases that were classified as other tumor types was fusion-positive. In one of the tumors with typical LGFMS appearance, we found that FUS was fused to the CREB3L1 gene instead of CREB3L2. The proteins encoded by these genes both belong to the same basic leucine-zipper family of transcription factors, and display extensive sequence homology in their DNA-binding domains. Thus, it is expected that the novel FUS/CREB3L1 chimera will have a similar impact at the cellular level as the much more common FUS/CREB3L2 fusion protein. Taken together, the results indicate that virtually all LGFMS are characterized by a chimeric FUS/CREB3L2 gene, and that rare cases may display a variant FUS/CREB3L1 fusion
Keywords
Adolescent/Adult/Aged/Amino Acid Sequence/Base Sequence/Child/Chromosomes,Human,Pair 16/genetics/Chromosomes,Human,Pair 7/Cyclic AMP Response Element-Binding Protein/Diagnosis,Differential/Female/Fibrosarcoma/Humans/Male/Middle Aged/Molecular Sequence Data/Nerve Tissue Proteins/RNA-Binding Protein FUS/Recombinant Fusion Proteins/Reverse Transcriptase Polymerase Chain Reaction/Soft Tissue Neoplasms/Transcription Factors/Translocation,Genetic/Tumor Markers,Biological
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2008 18:33
Last modification date
20/08/2019 15:27
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