Isolation and sequence of rat peptide YY and neuropeptide Y

Details

Serval ID
serval:BIB_B9ACE1C70FBF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Isolation and sequence of rat peptide YY and neuropeptide Y
Journal
Regulatory Peptides
Author(s)
Corder  R., Gaillard  R. C., Bohlen  P.
ISSN
0167-0115 (Print)
Publication state
Published
Issued date
06/1988
Volume
21
Number
3-4
Pages
253-61
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
Rat peptide YY and rat neuropeptide Y have been isolated in parallel from colon and brain extracts respectively, using salt fractionation, gel filtration chromatography, cation-exchange HPLC, and reverse phase phenyl-silica HPLC. Immunoreactivity was identified using a combination of 3 NPY immunoassays which exhibit differing cross-reactivities for PYY (90%, less than 0.01% and 30% respectively). The yield at the final purification step was 1.2 nmol rPYY and 0.5 nmol rNPY. Half of each purified peptide was subjected to complete microsequence analysis. This showed that while rat NPY was structurally identical to human NPY, the sequence of PYY from rat colon was the same as porcine PYY isolated from extracts of duodenum.
Keywords
Amino Acid Sequence Animals Chromatography, Gel Chromatography, High Pressure Liquid Gastrointestinal Hormones/*isolation & purification Immunoassay/methods Molecular Sequence Data Neuropeptide Y/*isolation & purification Peptide YY Peptides/*isolation & purification Radiometry Rats
Pubmed
Web of science
Create date
15/02/2008 16:57
Last modification date
20/08/2019 15:27
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