Cycles of gene expression and genome response during mammalian tissue regeneration.

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State: Public
Version: Final published version
Serval ID
serval:BIB_B98F4A2BF637
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cycles of gene expression and genome response during mammalian tissue regeneration.
Journal
Epigenetics & chromatin
Author(s)
Rib L., Villeneuve D., Minocha S., Praz V., Hernandez N., Guex N., Herr W.
Working group(s)
CycliX Consortium
Contributor(s)
Hernandez N., Delorenzi M., Deplancke B., Desvergne B., Guex N., Herr W., Naef F., Rougemont J., Schibler U., Andersin T., Cousin P., Gilardi F., Gos P., Lammers F., Lopes M., Mange F., Minocha S., Raghav S., Villeneuve D., Fabbretti R., Vlegel V., Xenarios I., Migliavacca E., Praz V., David F., Jarosz Y., Kuznetsov D., Liechti R., Martin O., Delafontaine J., Cajan J., Carmeli C., Gustafson K., Krier I., Leleu M., Molina N., Naldi A., Rib L., Sobel J., Symul L., Bounova G., Jacquet P.
ISSN
1756-8935 (Electronic)
ISSN-L
1756-8935
Publication state
Published
Issued date
12/09/2018
Peer-reviewed
Oui
Volume
11
Number
1
Pages
52
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Compensatory liver hyperplasia-or regeneration-induced by two-thirds partial hepatectomy (PH) permits the study of synchronized activation of mammalian gene expression, particularly in relation to cell proliferation. Here, we measured genomic transcriptional responses and mRNA accumulation changes after PH and sham surgeries.
During the first 10-20 h, the PH- and sham-surgery responses were very similar, including parallel early activation of cell-division-cycle genes. After 20 h, however, whereas post-PH livers continued with a robust and coordinate cell-division-cycle gene-expression response before returning to the resting state by 1 week, sham-surgery livers returned directly to a resting gene-expression state. Localization of RNA polymerase II (Pol II), and trimethylated histone H3 lysine 4 (H3K4me3) and 36 (H3K36me3) on genes dormant in the resting liver and activated during the PH response revealed a general de novo promoter Pol II recruitment and H3K4me3 increase during the early 10-20 h phase followed by Pol II elongation and H3K36me3 accumulation in gene bodies during the later proliferation phase. H3K36me3, generally appearing at the first internal exon, was preceded 5' by H3K36me2; 3' of the first internal exon, in about half of genes H3K36me3 predominated and in the other half H3K36me2 and H3K36me3 co-existed. Further, we observed some unusual gene profiles with abundant Pol II but little evident H3K4me3 or H3K36me3 modification, indicating that these modifications are neither universal nor essential partners to Pol II transcription.
PH and sham surgical procedures on mice reveal striking early post-operatory gene expression similarities followed by synchronized mRNA accumulation and epigenetic histone mark changes specific to PH.
Keywords
Animals, DNA Polymerase II/metabolism, Epigenesis, Genetic, Histone Code, Liver/metabolism, Liver/physiology, Liver Regeneration, Male, Mice, Mice, Inbred C57BL, Promoter Regions, Genetic, Transcriptional Activation, Transcriptome, Gene expression, Histone modification, Liver regeneration, Partial hepatectomy, Transcription
Pubmed
Web of science
Open Access
Yes
Create date
19/09/2018 13:30
Last modification date
19/02/2020 11:54
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