The identification of tyrosine as a common key residue in unrelated H-2Kd restricted antigenic peptides

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Serval ID
serval:BIB_B9764698CBB6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The identification of tyrosine as a common key residue in unrelated H-2Kd restricted antigenic peptides
Journal
International Immunology
Author(s)
Maryanski  J. L., Romero  P., Van Pel  A., Boon  T., Salemme  F. R., Cerottini  J. C., Corradin  G.
ISSN
0953-8178 (Print)
Publication state
Published
Issued date
10/1991
Volume
3
Number
10
Pages
1035-42
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Abstract
We have compared the activity of several Kd- or Ld-restricted antigenic peptides as competitors in a functional competition assay using cytolytic T lymphocyte (CTL) clones. All of four unrelated Kd-restricted peptides tested could compete with each other but not with the Ld-restricted peptide P91A-. 12-24 (P91A). Moreover, the P91A peptide failed to compete with the four Kd-restricted peptides. In contrast, another Ld-restricted peptide [mouse cytomegalovirus (MCMV) pp89 167-176] could clearly compete with both Kd- and Ld-restricted peptides. The comparison of a series of modified MCMV pp89 peptides suggested that distinct structural features allow the interaction of the peptide with the two different MHC class I molecules. We showed previously that the competitor activity of two different Kd-restricted antigenic peptides was reduced substantially upon Ala substitution of the single Tyr residues present in these peptides. We now show a similar effect for two additional Kd-restricted peptides. Our results thus suggest that Tyr may function as an 'anchor' residue for many antigenic peptides that bind to the Kd molecule. Molecular modeling of the presumed antigen-binding site of the Kd molecule revealed the presence of two deep cavities that may be involved in binding peptide amino acid side chains. A model illustrating one possible interaction of a Tyr-containing peptide with the Kd molecule is presented.
Keywords
Alanine/immunology Amino Acid Sequence Animals Binding Sites Binding, Competitive Epitopes/*immunology Genes, MHC Class I/immunology Major Histocompatibility Complex/*immunology Mice Models, Molecular Molecular Sequence Data Oligopeptides/*immunology T-Lymphocytes, Cytotoxic/*immunology Tyrosine/*immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:14
Last modification date
01/10/2019 6:19
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