Restoration of growth potential in paraclones of human keratinocytes by a viral oncogene

Details

Serval ID
serval:BIB_B95747560B32
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Restoration of growth potential in paraclones of human keratinocytes by a viral oncogene
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Barrandon  Y., Morgan  J. R., Mulligan  R. C., Green  H.
ISSN
0027-8424 (Print)
Publication state
Published
Issued date
06/1989
Volume
86
Number
11
Pages
4102-6
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jun
Abstract
Human diploid keratinocytes may be divided into three clonal types with differing capacities for proliferation. The paraclone, which has the shortest life span, is limited to 15 divisions, after which all the cells undergo programmed terminal differentiation. By means of a retroviral vector, paraclones which have not completed their life span and which consist of not more than a few hundred cells can be transduced at a high frequency with DNA complementary to the 12S transcript of the adenovirus early region 1A gene. Transformation can be detected within a single cultivation by the formation of progressively growing colonies. The transformants appear to have an unlimited growth potential, and they form a disorganized epidermis when they are grafted as an epithelium onto athymic mice. These experiments clearly show that, in order to be transformed by a viral oncogene, the target cell need not be a stem cell.
Keywords
Aged Animals Cell Division *Cell Transformation, Viral Cells, Cultured Clone Cells Epidermis/*cytology/transplantation Female Humans Immunoblotting Keratins/metabolism Mice Mice, Nude *Oncogenes Retroviridae/*genetics Transplantation, Heterologous
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 8:41
Last modification date
20/08/2019 15:27
Usage data