Resistance of different Chlamydia-like organisms to quinolones and mutations in the quinoline resistance-determining region of the DNA gyrase A- and topoisomerase-encoding genes
Details
Serval ID
serval:BIB_B94CBD25CAEF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Resistance of different Chlamydia-like organisms to quinolones and mutations in the quinoline resistance-determining region of the DNA gyrase A- and topoisomerase-encoding genes
Journal
International Journal of Antimicrobial Agents
ISSN
0924-8579 (Print)
Publication state
Published
Issued date
06/2006
Volume
27
Number
6
Pages
541-4
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
Parachlamydia acanthamoebae has been shown to be resistant to fluoroquinolones. In this study, we determined the susceptibility of Neochlamydia hartmannellae and Simkania negevensis to quinolones using amoebal co-culture in the presence of serial dilutions of ciprofloxacin. The gyrA and parC quinoline resistance-determining regions (QRDRs) were amplified and sequenced, and amino acid substitutions that may explain the observed quinolone resistance were investigated. Neochlamydia and Simkania were resistant to 16 microg/mL ciprofloxacin. Substitution at positions 83 and 70 of GyrA QRDR were present in Neochlamydia and in both Parachlamydia strains studied. For Simkania, substitutions at positions 99 and 101 in the GyrA QRDR and at positions 81 and 84 in the ParC QRDR were observed. Resistance of Parachlamydia, Neochlamydia and Simkania to quinolones is likely due to these mutations.
Keywords
Amino Acid Sequence
Anti-Infective Agents/*pharmacology
Chlamydia/*drug effects/genetics
Ciprofloxacin/*pharmacology
DNA Gyrase/chemistry/*genetics
DNA Topoisomerase IV/chemistry/*genetics
Drug Resistance, Bacterial
Molecular Sequence Data
Structure-Activity Relationship
Pubmed
Web of science
Create date
25/01/2008 14:28
Last modification date
20/08/2019 15:27