Impedance-Based Single-Cell Pipetting.

Details

Serval ID
serval:BIB_B9267A9F190F
Type
Article: article from journal or magazin.
Collection
Publications
Title
Impedance-Based Single-Cell Pipetting.
Journal
SLAS technology
Author(s)
Bonzon D., Muller G., Bureau J.B., Uffer N., Beuchat N., Barrandon Y., Renaud P.
ISSN
2472-6311 (Electronic)
ISSN-L
2472-6303
Publication state
Published
Issued date
06/2020
Peer-reviewed
Oui
Volume
25
Number
3
Pages
222-233
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here, we report the modeling, designing, and testing of a disposable pipet tip integrating a cell sensor based on the Coulter principle. We investigate, test, and discuss the effects of design parameters on the sensor performances with an analytical model. We also describe a system that enables the dispensing of single cells using an instrumented pipet coupled with the sensing tip. Most importantly, this system allows the recording of an impedance trace to be used as proof of single-cell isolation. We assess the performances of the system with beads and cells. Finally, we show that the electrical detection has no effect on cell viability.
Keywords
3T3 Cells, Animals, Cell Membrane/metabolism, Cell Survival, Electric Impedance, Electrodes, Mice, Particle Size, Reproducibility of Results, Single-Cell Analysis, dispensing, disposable pipet tip, impedance spectroscopy, single cell
Pubmed
Web of science
Open Access
Yes
Create date
02/04/2020 17:14
Last modification date
05/04/2024 16:34
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