Developmental expression of sodium entry pathways in rat nephron

Details

Serval ID
serval:BIB_B90B1600B5C9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Developmental expression of sodium entry pathways in rat nephron
Journal
American Journal of Physiology
Author(s)
Schmitt  R., Ellison  D. H., Farman  N., Rossier  B. C., Reilly  R. F., Reeves  W. B., Oberbaumer  I., Tapp  R., Bachmann  S.
ISSN
0363-6127
Publication state
Published
Issued date
03/1999
Volume
276
Number
3 Pt 2
Pages
F367-81
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar
Abstract
During the past several years, sites of expression of ion transport proteins in tubules from adult kidneys have been described and correlated with functional properties. Less information is available concerning sites of expression during tubule morphogenesis, although such expression patterns may be crucial to renal development. In the current studies, patterns of renal axial differentiation were defined by mapping the expression of sodium transport pathways during nephrogenesis in the rat. Combined in situ hybridization and immunohistochemistry were used to localize the Na-Pi cotransporter type 2 (NaPi2), the bumetanide-sensitive Na-K-2Cl cotransporter (NKCC2), the thiazide-sensitive Na-Cl cotransporter (NCC), the Na/Ca exchanger (NaCa), the epithelial sodium channel (rENaC), and 11beta-hydroxysteroid dehydrogenase (11HSD). The onset of expression of these proteins began in post-S-shape stages. NKCC2 was initially expressed at the macula densa region and later extended into the nascent ascending limb of the loop of Henle (TAL), whereas differentiation of the proximal tubular part of the loop of Henle showed a comparatively retarded onset when probed for NaPi2. The NCC was initially found at the distal end of the nascent distal convoluted tubule (DCT) and later extended toward the junction with the TAL. After a period of changing proportions, subsegmentation of the DCT into a proximal part expressing NCC alone and a distal part expressing NCC together with NaCa was evident. Strong coexpression of rENaC and 11HSD was observed in early nascent connecting tubule (CNT) and collecting ducts and later also in the distal portion of the DCT. Ontogeny of the expression of NCC, NaCa, 11HSD, and rENaC in the late distal convolutions indicates a heterogenous origin of the CNT. These data present a detailed analysis of the relations between the anatomic differentiation of the developing renal tubule and the expression of tubular transport proteins.
Keywords
11-beta-Hydroxysteroid Dehydrogenases Aging/*metabolism Animals Animals, Newborn/growth & development/metabolism Carrier Proteins/metabolism Epithelial Sodium Channel Hydroxysteroid Dehydrogenases/metabolism Nephrons/growth & development/*metabolism Rats Rats, Sprague-Dawley Sodium/*metabolism Sodium Channels/metabolism Sodium-Calcium Exchanger/metabolism Sodium-Phosphate Cotransporter Proteins Sodium-Phosphate Cotransporter Proteins, Type II Sodium-Potassium-Chloride Symporters *Symporters
Pubmed
Web of science
Create date
24/01/2008 13:00
Last modification date
20/08/2019 15:27
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