Metabolic and structural rearrangement during dark-induced autophagy in soybean (Glycine max L.) nodules: An electron microscopy and P-31 and C-13 nuclear magnetic resonance study

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Version: author
Serval ID
serval:BIB_B8E99A724219
Type
Article: article from journal or magazin.
Collection
Publications
Title
Metabolic and structural rearrangement during dark-induced autophagy in soybean (Glycine max L.) nodules: An electron microscopy and P-31 and C-13 nuclear magnetic resonance study
Journal
Planta
Author(s)
Vauclare P., Bligny R., Gout E., De Meuron V., Widmer F.
ISSN
0032-0935
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
231
Number
6
Pages
1495-1504
Language
english
Abstract
The effects of dark-induced stress on the evolution of the soluble metabolites present in senescent soybean (Glycine max L.) nodules were analysed in vitro using C-13- and P-31-NMR spectroscopy. Sucrose and trehalose were the predominant soluble storage carbons. During dark-induced stress, a decline in sugars and some key glycolytic metabolites was observed. Whereas 84% of the sucrose disappeared, only one-half of the trehalose was utilised. This decline coincides with the depletion of Gln, Asn, Ala and with an accumulation of ureides, which reflect a huge reduction of the N-2 fixation. Concomitantly, phosphodiesters and compounds like P-choline, a good marker of membrane phospholipids hydrolysis and cell autophagy, accumulated in the nodules. An autophagic process was confirmed by the decrease in cell fatty acid content. In addition, a slight increase in unsaturated fatty acids (oleic and linoleic acids) was observed, probably as a response to peroxidation reactions. Electron microscopy analysis revealed that, despite membranes dismantling, most of the bacteroids seem to be structurally intact. Taken together, our results show that the carbohydrate starvation induced in soybean by dark stress triggers a profound metabolic and structural rearrangement in the infected cells of soybean nodule which is representative of symbiotic cessation.
Keywords
Peribacteroid membrane, Glycine, Metabolic NMR, Nodules, Senescence, HIGHER-PLANT CELLS, FIXING LEGUME NODULES, ROOT-NODULES, PERIBACTEROID MEMBRANE, SYMBIOTIC MEMBRANES, SUCROSE DEPRIVATION, UREIDE DEGRADATION, INDUCED SENESCENCE, GLYOXYLATE CYCLE, ACID-COMPOSITION
Pubmed
Web of science
Create date
13/08/2015 8:54
Last modification date
20/08/2019 16:26
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