Antibodies to lipopolysaccharides after immunization of humans with the rough mutant Escherichia coli J5

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Serval ID
serval:BIB_B8D897551E74
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Antibodies to lipopolysaccharides after immunization of humans with the rough mutant Escherichia coli J5
Journal
Journal of Infectious Diseases
Author(s)
Baumgartner  J. D., Heumann  D., Calandra  T., Glauser  M. P.
ISSN
0022-1899
Publication state
Published
Issued date
04/1991
Peer-reviewed
Oui
Volume
163
Number
4
Pages
769-72
Language
english
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Abstract
To investigate whether immunization with Escherichia coli J5 boiled cells induces antibodies directed at deep core structures, antibodies against J5 lipopolysaccharide (LPS), Re LPS, and lipid A were measured in the serum of 70 volunteers before and 2 weeks after immunization. To improve the sensitivity and the specificity of ELISA, complexes of core LPS with high-density lipoproteins were used instead of free core LPS as antigens. A median three-fold increase in antibodies directed against J5 LPS was observed, but no significant increase in the antibodies against Re LPS or lipid A was found. Since J5 antiserum did not react with several smooth LPS or with Re LPS and lipid A, cross-reactivity could not be demonstrated. Thus, immunization of volunteers with E. coli J5 produced a modest specific antibody response against J5 LPS. The mechanism of protection previously observed with J5 antiserum remains unclear.
Keywords
Antibodies, Bacterial/*biosynthesis/blood Bacterial Vaccines/*immunology Enzyme-Linked Immunosorbent Assay Escherichia coli/*immunology Humans Immune Sera/immunology *Immunization Immunoglobulin G/biosynthesis Immunoglobulin M/biosynthesis Lipopolysaccharides/*immunology Lipoproteins, HDL/immunology
Pubmed
Web of science
Open Access
Yes
Create date
19/01/2008 18:12
Last modification date
01/10/2019 6:19
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