Bipolar resistivity profiling of 3D tissue culture

Details

Serval ID
serval:BIB_B8D64B194F49
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Bipolar resistivity profiling of 3D tissue culture
Journal
Biosensors and Bioelectronics
Author(s)
Linderholm  P., Vannod  J., Barrandon  Y., Renaud  P.
ISSN
0956-5663 (Print)
Publication state
Published
Issued date
01/2007
Volume
22
Number
6
Pages
789-96
Notes
Evaluation Studies
Journal Article --- Old month value: Jan 15
Abstract
We describe a new low-cost technique for continuous monitoring of the thickness of biofilms and tissue cultures, and we demonstrate the advantage of using electrodes of different dimensions to probe different depths of a sample. We have used electric impedance spectroscopy to monitor keratinocyte stem cells (YF29) growing on an array of Ti/Pt coplanar microelectrodes. The thickness of the sample was reconstructed by fitting the measurements to theoretical curves. We have developed an algorithm for the rapid calculation of the resistance through a multilayered sample. This algorithm is based on conformal mapping and the serial partial capacitance technique. The validity of the technique was tested by measuring the sedimentation rate of an alumina powder. Sample thicknesses between 10 and 80 microm could be measured with a resolution of a few microns using the device.
Keywords
Biological Assay/instrumentation/*methods Biosensing Techniques/instrumentation/*methods Cell Culture Techniques/instrumentation/*methods Cell Line Electric Impedance Electrochemistry/instrumentation/*methods Humans Keratinocytes/*physiology *Microelectrodes Plethysmography, Impedance/instrumentation/*methods
Pubmed
Web of science
Create date
28/01/2008 8:41
Last modification date
20/08/2019 15:26
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