Susceptibility of B-cell deficient C57BL/6 (microMT) mice to Neospora caninum infection

Details

Serval ID
serval:BIB_B8C8A8677555
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Susceptibility of B-cell deficient C57BL/6 (microMT) mice to Neospora caninum infection
Journal
Parasite Immunology
Author(s)
Eperon  S., Bronnimann  K., Hemphill  A., Gottstein  B.
ISSN
0141-9838
Publication state
Published
Issued date
05/1999
Peer-reviewed
Oui
Volume
21
Number
5
Pages
225-36
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
Neospora caninum is a coccidian parasite of veterinary importance by causing abortion or stillbirth in cattle among other problems in diverse animal species. We assessed an experimental murine model for its suitability to study the immune response to N. caninum infection. Thus, wild-type (wt) C57BL/6 mice and B-cell (and consequently antibody)-deficient microMT mice were infected with N. caninum tachyzoites and sacrificed at days 10, 24 and 29-44 post infection (dpi). Various organs were collected for parasitological and pathological analysis, spleen and serum for immunological investigations. Splenocytes were in vitro-stimulated with N. caninum (NC)- and T. gondii-antigens for assessing T cell proliferation and cytokine production. While wt mice were resistant to disease, microMT mice died starting from 29 dpi onwards. Histological examination of brain tissue from microMT mice exhibited a high infection intensity with multifocal necrotic cerebral lesions, which were absent in the brains of wt mice. NC antigen-stimulated spleen cells of both wt and microMT mice infected with N. caninum showed a marked proliferative depression at 10 dpi. At 24 dpi, this immunosuppression was still maintained in microMT mice whereas it was restored in wt mice. Stimulated splenocytes of infected microMT mice secreted significantly less IFN-gamma and less IL-10 than corresponding wt splenocytes. For IL-10, this difference increased with time. The susceptibility of microMT mice appeared associated to B-cell deficiency, allowing the persistent spread of the parasite causing immunosuppression and finally resulting in a lethal outcome of infection.
Keywords
Animals Antibodies, Protozoan/blood Antigens, Protozoan/*immunology B-Lymphocytes/*immunology Cercopithecus aethiops Coccidiosis/*immunology Disease Models, Animal Immunoglobulin G/blood Interferon Type II/analysis Interleukin-10/analysis Mice Mice, Inbred C57BL Mice, Knockout Neospora/*immunology Specific Pathogen-Free Organisms Vero Cells
Pubmed
Web of science
Create date
17/01/2008 13:27
Last modification date
20/08/2019 15:26
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