Comparison of the proliferative activity of neuroblasts from chick embryo cerebral hemispheres of different ages in culture.

Details

Serval ID
serval:BIB_B8AF79374C3B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Comparison of the proliferative activity of neuroblasts from chick embryo cerebral hemispheres of different ages in culture.
Journal
Developmental neuroscience
Author(s)
Barakat I., Sensenbrenner M.
ISSN
0378-5866
Publication state
Published
Issued date
1988
Peer-reviewed
Oui
Volume
10
Number
3
Pages
173-179
Language
english
Abstract
Dissociated cerebral hemisphere cells from 4- to 7-day-old chick embryos were cultured either on a collagen or a polylysine substrate in a serum-containing medium. Neurons were characterized by the demonstration of acetylcholinesterase, the presence of D2/N-CAM glycoprotein and neurofilament proteins. The proliferation of neuronal precursor cells was shown by morphological observations, autoradiographic analysis and measurements of [3H]-thymidine incorporation. Neuronal precursors derived from the 6-day-old embryos showed the highest proliferative activity. Neuroblast proliferation was found to be dependent on the culture substrates (i.e. polylysine or collagen), which yielded either isolated cells or cell aggregates, and the latter favored the mitogenic effect.
Keywords
Acetylcholinesterase, Animals, Antigens, Surface, Cell Adhesion Molecules, Cell Division, Cerebral Cortex, Chick Embryo, Intermediate Filament Proteins, Neurofilament Proteins, Neurons, Thymidine, Time Factors
Pubmed
Web of science
Create date
30/03/2009 9:31
Last modification date
20/08/2019 15:26
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