Development of cholinergic properties in nerve cell cultures in the presence of brain extract.

Details

Serval ID
serval:BIB_B88A800F39EC
Type
Article: article from journal or magazin.
Collection
Publications
Title
Development of cholinergic properties in nerve cell cultures in the presence of brain extract.
Journal
Brain research
Author(s)
Barakat I., Massarelli R., Courageot J., Devilliers G., Sensenbrenner M.
ISSN
0006-8993
Publication state
Published
Issued date
1983
Peer-reviewed
Oui
Volume
279
Number
1-2
Pages
207-216
Language
english
Abstract
Cells dissociated from cerebral hemispheres of 6-day-old chick embryos were cultured either in standard nutrient medium or in the presence of a brain extract from 8-day-old chick embryo. Morphological observations showed the development of bipolar and multipolar neurons in both culture conditions and acetylcholinesterase activity was found in all neuronal cells. Brain extract stimulated the morphological maturation of neurons, expressed by the formation of fiber bundles, fine structural maturation and development of synapses rich in clear vesicles. Furthermore, acetylcholinesterase and choline acetyltransferase activities were higher in the cultures treated with brain extract. In these cultures, the values of choline acetyltransferase activity reached a peak at 10 days and then decreased. These observations are discussed with particular reference to proliferation, maturation and degeneration of cholinergic neurons.
Keywords
Acetylcholinesterase, Animals, Brain, Cell Differentiation, Chick Embryo, Choline O-Acetyltransferase, Cholinergic Fibers, Culture Media, Culture Techniques, Nerve Degeneration, Nerve Growth Factors, Neurons
Pubmed
Web of science
Create date
30/03/2009 9:35
Last modification date
20/08/2019 15:26
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